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#83527 PRDM14 (E1D5S) XP® Rabbit mAb

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2019年1月15日15時15分 現在
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#83527S100 μL61,000
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#83527T20 μL39,000
感度分子量 (kDa)抗体の由来貯法
内在性70Rabbit IgG-20℃
種交差性 (社内試験済)
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83527 の推奨プロトコール i

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特異性・感度
PRDM14 (E1D5S) XP® Rabbit mAb recognizes endogenous levels of total PRDM14 protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with full-length recombinant protein specific to human PRDM14 protein.

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社内データ

※下記の社内データは、すべて83527 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PRDM14 (E1D5S) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, MCF7 and 293T are negative for PRDM14 expression.

IP

IP

Immunoprecipitation of PRDM14 from NCCIT cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PRDM14 (E1D5S) XP® Rabbit mAb. Western blot analysis was performed using PRDM14 (E1D5S) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCCIT cells (left, positive) and 293T cells (right, negative) using PRDM14 (E1D5S) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NCCIT cells (green) and 293T cells (blue) using PRDM14 (E1D5S) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either PRDM14 (E1D5S) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, human ASXL1 upstream primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

PR domain zinc finger protein 14 (PRDM14) is a likely protein lysine methyltransferase that is primarily expressed in primordial germ cells and pluripotent embryonic stem cells. It is essential for the establishment and maintenance of primordial germ cells and critical for the maintenance of pluripotency in embryonic stem cells (1-3). PRDM14 represses genes involved in the differentiation of stem cells into various cell lineages, likely via a combination of interactions with TET proteins, the polycomb repressive complex 2 (PRC2), and CBFA2T2 (3-8). In addition, overexpression of PRDM14 in combination with Jarid2 promotes induced pluripotent stem cell (iPSC) formation (9). PRDM14 protein levels are overexpressed in certain cancers, including breast, leukemia (T-ALL), and non-small cell lung cancer (NSCLC) (10-13), and PRDM14 overexpression may serve as a novel prognostic marker in NSCLC (14). Targeting PRDM14 overexpression with a siRNA-based therapy was shown to decrease liver metastasis in a murine pancreatic cancer model, suggesting potential as a therapeutic option for cancers where this protein is abnormally expressed (15).

使用例
 
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Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

本製品は試験研究用です。

PRDM14 (E1D5S) XP® Rabbit mAb

Immune Cell Signaling Pathways

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