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#8353 DUB Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#8353T1 Kit115,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-CYLD (Ser418) Antibody #4500 20 µl WB H M, R 108 Rabbit
STAMBP Antibody #5245 20 µl WB, IP H, Mk 50 Rabbit
A20/TNFAIP3 (D13H3) Rabbit mAb #5630 20 µl WB, IP H, M, R, Mk 82 Rabbit IgG
UCHL1 (D3T2E) XP® Rabbit mAb #13179 20 µl WB, IHC-P, IF-IC, F H, M, R, Mk Hm, B, Dg, Pg, Hr 27 Rabbit IgG
HAUSP (D17C6) XP® Rabbit mAb #4833 20 µl WB, IP, IF-IC H, M, R, Mk 135, 140 Rabbit IgG
USP9X Antibody #5751 20 µl WB H, M, R, Mk, Dg 270 Rabbit
CYLD (D1A10) Rabbit mAb #8462 20 µl WB, IP H, M, R, Mk Hm, B, Dg, GP, Hr 108 Rabbit IgG
UCHL3 (D25E6) Rabbit mAb #8141 20 µl WB H, M, R B, Dg, Hr 27 Rabbit IgG
USP10 (D7A5) Rabbit mAb #8501 20 µl WB, IP, IF-IC H, M, R, Mk 110 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, Mk=Monkey, M=Mouse, R=Rat, Dg=Dog

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HAUSP (D17C6) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or treated with hTNF-α #8902 (20 ng/ml for 20 minutes), using Phospho-CYLD (Ser418) Antibody (upper) or CYLD Antibody #4495 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using STAMBP Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from G-361, JJN-3, and A20 cell lines using A20/TNFAIP3 (D13H3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP9X Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UCHL3 (D25E6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CYLD (D1A10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP10 (hUSP10-Myc/DDK, +), using USP10 (D7A5) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UCHL1 (D3T2E) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using HAUSP (D17C6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 (40 nM) and A23187 (2 μM) for various amounts of time, using A20/TNFAIP3 (D13H3) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human UCHL3 (hUCHL3, +), using UCHL3 (D25E6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP10 (D7A5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human UCHL1 (hUCHL1-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL3 (hUCHL3-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL5 (hUCHL5-Myc/DDK; +), and Myc/DDK-tagged full-length human BAP1 (hBAP1-Myc/DDK; +), using UCHL1 (D3T2E) XP® Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with a Myc-DDK-tagged human A20/TNFAIP3 construct (+), using A20/TNFAIP3 (D13H3) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT 116 cells using USP10 (D7A5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos and K-562 cells using UCHL1 (D3T2E) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, UCHL1 protein expression is not detected in K-562 cells.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using UCHL1 (D3T2E) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded DU 145 (left; positive) and LNCaP (right; negative) cell pellets using UCHL1 (D3T2E) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using UCHL1 (D3T2E) XP® Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using UCHL1 (D3T2E) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells (blue) and Ramos cells (green) using UCHL1 (D3T2E) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of DU 145 (positive; left) and LNCaP (negative; right) cells using UCHL1 (D3T2E) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).


バックグラウンド

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). CYLD deubiquitinase regulates inflammation and cell proliferation by down regulating NF-κB signaling through removal of ubiquitin chains from several NF-κB pathway proteins (3,4). Phosphorylation at Ser418 decreases CYLD deubiquitinase activity and is important for IKKε-driven transformation (5). STAM-binding protein (STAMBP or AMSH) is an endosomal DUB that preferentially displays ubiquitin isopeptidase activity toward K63-linked chains (6,7). The amino-terminus of A20 contains deubiquitinating activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase activity for Lys48 branches of the same substrates and leads to their degradation (8). Both enzymes have been implicated in neurodegenerative diseases (9-11) and play a role in the regulation of neuronal development and spermatogenesis (10,13,14). UCHL1 binds monoubiquitin and UCHL3 shows affinity for both ubiquitin and NEDD8, a ubiquitin-like molecule (11,12). HAUSP can bind and deubiquitinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins (15,16). HAUSP also modifies other ubiquitinated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR (17,18). USP10 appears to be regulated through both protein-protein interactions and phosphorylation. Interaction of USP10 with Ras-GAP SH3 domain binding protein (G3BP) inhibits its ability to disassemble ubiquitin chains (19). ATM-mediated phosphorylation at Thr42 and Ser337 stabilizes USP10, promoting redistribution from the cytoplasm to the nucleus, where it functions in p53 deubiquitination, stabilization, and activation in response to genotoxic stress (20). USP9X possesses a well-conserved catalytic domain with cysteine peptidase activity, which allows for cleavage of ubiquitin and polyubiquitin conjugates. While USP9X expression has been shown to be critical for normal mammalian development (21-23), many of its substrates are only beginning to be elucidated.

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

DUB Antibody Sampler Kit

Immune Cell Signaling Pathways

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