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#8356 Death Receptor Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#8356T1 Kit115,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Fas (C18C12) Rabbit mAb #4233 20 µl WB, IHC-P H 40-50 Rabbit IgG
TNF-R1 (C25C1) Rabbit mAb #3736 20 µl WB, IP H 55 Rabbit IgG
TNF-R2 Antibody #3727 20 µl WB, IP H, M, R Mk 65 Rabbit
DR5 (D4E9) XP® Rabbit mAb #8074 20 µl WB, IP, IF-IC H 40, 48 Rabbit IgG
DcR2 (D13H4) Rabbit mAb #8049 20 µl WB, IP H, Mk 45-60 Rabbit IgG
DcR3 Antibody #4758 20 µl WB H, R 32 Rabbit
FADD Antibody (Human Specific) #2782 20 µl WB H 28 Rabbit
TRADD (7G8) Rabbit mAb #3684 20 µl WB, IP H 32 Rabbit IgG
RIP (D94C12) XP® Rabbit mAb #3493 20 µl WB, IP, IF-IC, F H, M, R, Hm, Mk 78 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HeLa cells treated with 4 mM hydroxyurea or 1 µg/ml nocodazole (each for 20 hours), using FADD Antibody (Human Specific).

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7, SR, and A673 cell lines, using TRADD (7G8) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of lysates from LN18, HeLa and HCT116 cell lines, using DcR3 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from SW620, NK-92 and SR cell lines, using TNF-R2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549, LN-18 and HeLa cells using TNF-R1 (C25C1) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, mock transfected or transfected with human Fas, and from ACHN and HT-1080 cell lines using Fas (C18C12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RIP (D94C12) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DR5 (D4E9) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human DcR2 (hDcR2; +), using DcR2 (D13H4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Fas (C18C12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected or transfected with human RIP construct, using RIP (D94C12) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, untreated or doxorubicin-treated (500 nM) for the indicated times, using DR5 (D4E9) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot anlaysis of extracts from various cell lines using DcR2 (D13H4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffn embedded human colon using Fas (C18C12) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 cells using RIP (D94C12) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with the long isoform of human DR5 (hDR5, +), using DR5 (D4E9) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Raji (positive, left) or K562 cells (negative, right) using Fas (C18C12) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of OVCAR8 cells using RIP (D94C12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-1080 cells using DR5 (D4E9) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC) resulting in activation of caspases. Death receptor signaling is also controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) which lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand (3-5). The RIP (receptor-interacting protein) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that can trigger pro-survival and inflammatory responses through the activation of NF-κB as well as pro-apoptotic pathways (6). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and for the recruitment to TNFR1 through interaction with TRADD (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (7,8). Caspase-8 dependent cleavage of the death domain on RIP can trigger the apoptotic activity of RIP (9). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (10,11).

使用文献
 
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3707   TNF-α Antibody
4756   DcR1 Antibody
4790   Caspase-8 (D35G2) Rabbit mAb
4927   Caspase-8 Antibody (Mouse Specific)
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7722   Blue Loading Buffer Pack
7723   Red Loading Buffer Pack
7727   Biotinylated Protein Ladder Detection Pack
9664   Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
9746   Caspase-8 (1C12) Mouse mAb
9915   Apoptosis Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Death Receptor Antibody Sampler Kit

Immune Cell Signaling Pathways

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