CST logo

#8358 Hedgehog Signaling Antibody Sampler Kit

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年3月25日15時35分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#8358T1 Kit84,000
ログインすると国内在庫状況がご確認いただけます。
8358 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Shh (C9C5) Rabbit mAb #2207 20 µl WB H, R, Z M, C, X 19, (45 kDa precursor) Rabbit IgG
PTCH1 (C53A3) Rabbit mAb #2468 20 µl WB, IP H 180-210 Rabbit IgG
PTCH2 (G1191) Antibody #2470 20 µl WB, IP H 130 Rabbit
SUFU (C54G2) Rabbit mAb #2520 20 µl WB H, M, R, Mk 54 Rabbit IgG
GLI1 (C68H3) Rabbit mAb #3538 20 µl WB, IP H 160 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, R=Rat, Z=Zebrafish, M=Mouse, Mk=Monkey

貯法
-20℃
社内データ

Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb #2207.

Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 construct, using PTCH1 (C53A3) Rabbit mAb #2468.

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing human PTCH2, using PTCH2 (G1191) Antibody #2470.


Western blot analysis of total cell lysates from various cell types using SUFU (C54G2) Rabbit mAb #2520.

Western blot analysis of extracts from RMS-13 and TOV-112D cells using GLI1 (V812) Antibody #2534.

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing human PTCH2, using PTCH2 (G1191) Antibody.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from various cell types using SUFU (C54G2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 expression construct, using PTCH1 (C53A3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from RMS-13 and TOV-112D cells using GLI1 (C68H3) Rabbit mAb.


IP

IP

Immunoprecipitation of endogenous PTCH1 from 293 cell lysates using PTCH1 (C53A3) Rabbit mAb. Western blot detection was performed using the same antibody.

バックグラウンド

The evolutionarily conserved Hedgehog (Hh) signaling pathway plays critical roles in the regulation of patterning, growth, and cell migration during embryonic development and adult tissue homeostasis. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh), and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the Hh receptors (6-9). The general organization of the Hh pathway consists of a series of repressive interactions. In the absence of Hh proteins (off-state), PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) (1,2). In the off-state, SUFU (Suppressor of Fused), originally identified in Drosophila as a suppressor of the Fused (Fu) kinase (10), suppresses Hh signaling by regulating the localization of the transcription factors Gli and Ci (11,12). In Drosophila, SUFU may also positively regulate Hh signaling depending on SUFU protein levels and Hh signal intensity (13).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2464   PTCH2 (L849) Antibody
2522   SUFU (C81H7) Rabbit mAb
2585   GLI2 (R770) Antibody
2643   GLI1 (L42B10) Mouse mAb
3538   GLI1 (C68H3) Rabbit mAb
6883   SignalFire™ ECL Reagent
7727   Biotinylated Protein Ladder Detection Pack

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Hedgehog Signaling Antibody Sampler Kit

Metabolic Reprogramming in Disease

お薦めリンク

テクニカルサポート

製品特集

Applications