CST logo

#84651 PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific)

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2018年11月15日15時25分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#84651S100 μL138,000
ログインすると国内在庫状況がご確認いただけます。
#84651T20 μL49,000

PDCD1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性40-75Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

マウス ラット、ハムスター
84651 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:200)免疫組織染色 (パラフィン) (1:200)免疫蛍光細胞染色 (IF-IC) (1:100)免疫蛍光染色 (IF-F) (1:100)フローサイトメトリー (1:100)Immunohistochemistry (Leica® Bond™) (1:100)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのPD-1 タンパク質を検出します。
使用抗原
マウスのPD-1 タンパク質のAla242 周辺領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて84651 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from EL4, CTLL-2, and NIH/3T3 using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of PD-1 protein from EL4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific). Western blot analysis was performed using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded mouse LL/2 syngeneic tumor using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) performed on the Leica® Bond™ Rx.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse ovary using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using PD-1 (D7D5W) XP® rabbit mAb (green), F4/80 (D2S9R) XP® rabbit mAb #70076 (orange), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD3ε (D4V8L) rabbit mAb #99940 (red), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan).


IF-F

IF-F

Confocal immunofluorescent analysis of mouse spleen using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DyLight 554™ Phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Note: PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) has been validated on freshly prepared frozen tissue sections that were fixed for 15 minutes in 4% formaldehyde. Over-fixation diminshes the performance of this antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of EL4 cells (positive, left) and NIH/3T3 cells (negative, right) using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of CTLL-2 cells (blue) and EL4 cells (green) using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


バックグラウンド

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).

使用例
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
6883   SignalFire™ ECL Reagent
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

本製品は試験研究用です。

PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific)

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications