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#85060 Phospho-Glucocorticoid Receptor (Ser134) (E9R9W) Rabbit mAb


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2019年3月25日15時35分 現在
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#85060S100 μL66,000

Glucocorticoid Receptor 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性91, 94Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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85060 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:100)


内在性レベルのSer134 がリン酸化されたGlucocorticoid Receptor タンパク質を検出します。ウェスタンブロッティングで、由来不明の200 kDa のタンパク質を検出することが確認されています。
ヒトのGlucocorticoid Receptor タンパク質のSer134 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて85060 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-), UV-treated (+), or UV-treated and subsequently treated with calf intestinal phosphatase (CIP) plus λ-phosphatase (+), using Phospho-Glucocorticoid Receptor (Ser134) (E9R9W) Rabbit mAb (upper) or Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 (lower).



Immunoprecipitation of Phospho-Glucocorticoid Receptor (Ser134) from UV-treated HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-Glucocorticoid Receptor (Ser134) (E9R9W) Rabbit mAb. Western blot analysis was performed using Phospho-Glucocorticoid Receptor (Ser134) (E9R9W) Rabbit mAb.


Glucocorticoid hormones control cellular proliferation, inflammation, and metabolism through their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors (1). GR is composed of several conserved structural elements, including a carboxy-terminal ligand-binding domain (which also contains residues critical for receptor dimerization and hormone-dependent gene transactivation), a neighboring hinge region containing nuclear localization signals, a central zinc-finger-containing DNA-binding domain, and an amino-terminal variable region that participates in ligand-independent gene transcription. In the absence of hormone, a significant population of GR is localized to the cytoplasm in an inactive form via its association with regulatory chaperone proteins, such as HSP90, HSP70, and FKBP52. On hormone binding, GR is released from the chaperone complex and translocates to the nucleus as a dimer to associate with specific DNA sequences termed glucocorticoid response elements (GREs), thereby enhancing or repressing transcription of specific target genes (2). It was demonstrated that GR-mediated transcriptional activation is modulated by phosphorylation (3-5). Although GR can be basally phosphorylated in the absence of hormone, it becomes hyperphosphorylated upon binding receptor agonists. It has been suggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor signaling, receptor stability, and receptor subcellular localization (3).

Phosphorylation of GR at Ser134 by p38 MAPK occurs in response to cellular stress and is hormone independent. The phosphorylation event results in stronger association with 14-3-3ζ, which alters chromatin binding and reduces GR transcriptional activity (6).

12041   Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb
3660   Glucocorticoid Receptor (D8H2) XP® Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-Glucocorticoid Receptor (Ser134) (E9R9W) Rabbit mAb

Immune Cell Signaling Pathways