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#85314 β-Amyloid Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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Amyloid beta 製品一覧

85314 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
APP Antibody #2452 20 µl WB H, M, R, Mk 100 to 140 Rabbit
β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb #12843 20 µl WB H M, R, Mk, B 4 Rabbit
β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb #12990 20 µl WB, IF-F H M, R, Mk, B 4 Rabbit IgG
β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb #12467 20 µl WB H M, R, Mk, B 4 Rabbit IgG
β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb #12077 20 µl WB H M, R, Mk, B 4 Rabbit IgG
β-Amyloid (D54D2) XP® Rabbit mAb #8243 20 µl WB, IP, IF-P H 5 Rabbit IgG
β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb #14975 20 µl WB, IHC-P, IF-F H 4 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen), IP=Immunoprecipitation, IF-P=Immunofluorescence (Paraffin), IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa (human), 3T3L1 (mouse) or C6 (rat) cells, untreated or nocodazole-treated (1 µg/ml, 16-18 hours), using APP Antibody.

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of human Aβ-42, Aβ-40, Aβ-39, Aβ-38, and Aβ-37 peptides (5 ng) using β-Amyloid (D54D2) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ-37 peptide using β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ-39 peptide using β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ-42 peptide using β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (2.5 ng) using β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

Western Blotting

Western Blotting

β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb specificity is demonstrated using peptide dot blot. β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb binds to pre-coated β-amyloid (pE3) peptide but not to β-amyloid (E3) peptide or scrambled β-amyloid (pE3) peptide.


Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ-42 (left) and Aβ-40 (right) peptides using β-Amyloid (D54D2) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (10 ng) using β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).


Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42 and Aβ-43 peptides (10 ng) using β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb (upper) and β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ (pE3-42) and Aβ (1-42) peptides using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (upper) and β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb #12843 (lower).

Western Blotting

Western Blotting

Western blot analysis of human Aβ-42 peptide (1 ng) and human cerebrospinal fluid (CSF) of an AD patient using β-Amyloid (D54D2) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (10 ng) using β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

IF-F

IF-F

Confocal immunofluorescent analysis of hippocampus from wild-type (left) and TG2576 (right) mice using β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.

IF-P

IF-P

Confocal immunofluorescent analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (D54D2) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Tg2576 mouse model of Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.


IF-F

IF-F

Confocal immunofluorescent analysis of wild-type (left) and Tg2576 mouse model of Alzheimer's brain (right) using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains an amyloid domain, which can be processed and released by two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). Several fragments corresponding to progressive APP processing at alternative cleavage sites have been identified (2). These include Aβ (1-37), Aβ (1-39), Aβ (1-40), and Aβ (1-42) (2). These fragments can also be N-terminally modified to generate pyroglutamate-3 Aβ (pE3-peptide) (3). Fragment-specific and pan-Aβ antibodies are used to detect and examine relative levels of individual Aβ fragments.

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
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5676   GSK-3α/β (D75D3) Rabbit mAb
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DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

β-Amyloid Antibody Sampler Kit

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