CST logo

#8572 Neuronal Marker IF Antibody Sampler Kit

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年1月17日15時10分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#8572T1 Kit84,000
ログインすると国内在庫状況がご確認いただけます。
8572 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
GFAP (D1F4Q) XP® Rabbit mAb #12389 20 µl WB, IF-F H, M, R 50 Rabbit IgG
CNPase (D83E10) XP® Rabbit mAb #5664 20 µl WB, IP, IF-F H, M, R 47 Rabbit IgG
β3-Tubulin (D71G9) XP® Rabbit mAb #5568 20 µl WB, IP, IF-F, IF-IC H, M, R 55 Rabbit IgG
Nestin (Rat-401) Mouse mAb #4760 20 µl IHC-P, IF-F R Mouse IgG1
Neurofilament-L (C28E10) Rabbit mAb #2837 20 µl WB, IHC-P, IF-F H, M, R 70 Rabbit IgG

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen), IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat

貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat brain, P1 (left) or P14 (right), using Nestin (Rat-401) Mouse mAb (top) or Neurofilament-L (C28E10) Rabbit mAb #2837 (lower). Note decreased Nestin staining as NFL staining increases in the developing rat brain.

Western Blotting

Western Blotting

Western blot analysis of HeLa and human cerebellum using β3-Tubulin (D71G9) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various brain tissues using CNPase (D83E10) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using GFAP (D1F4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb.


IF-F

IF-F

Confocal immunofluorescent analysis of P1 rat brain (left) and adult rat brain (right) using Nestin (Rat-401) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of P19 cells that were differentiated with retinoic acid, using β3-Tubulin (D71G9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 #4084® (fluorescent DNA dye).


IF-F

IF-F

Confocal immunofluorescent analysis of rat cerebellum using GFAP (D1F4Q) XP® Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse cerebellum using β3-Tubulin (D71G9) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-F

IF-F

Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The antibodies in this kit serve as neuronal markers to determine protein localization in neurons. The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (2). Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells (3). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (4). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization and oligodendrocyte process outgrowth (5). GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used by investigators as a marker for intracranial and intraspinal tumors arising from astrocytes (6).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
4084   DRAQ5®
4408   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
4409   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4410   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
4412   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
4413   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4414   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
8889   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)
8890   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)
8961   ProLong® Gold Antifade Reagent with DAPI
9071   ProLong® Gold Antifade Reagent
9999   Nonfat Dry Milk

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Neuronal Marker IF Antibody Sampler Kit

Metabolic Reprogramming in Disease

お薦めリンク

テクニカルサポート

製品特集

Applications