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#8575 Calcium Ion Regulation Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#8575T1 Kit92,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
ATP2A2/SERCA2 (D51B11) Rabbit mAb #9580 20 µl WB H, M, R, Mk 114, 140 Rabbit IgG
Phospho-Phospholamban (Ser16/Thr17) Antibody #8496 20 µl WB R H, M, B, Dg, Pg 6 (monomer); 12, 24 (oligomers) Rabbit
Phospholamban (D9W8M) Rabbit mAb #14562 20 µl WB H, M, R 12, 24 Rabbit IgG
Phospho-PKA C (Thr197) (D45D3) Rabbit mAb #5661 20 µl WB H, M, R, Mk 42 Rabbit IgG
PKA C-α (D38C6) Rabbit mAb #5842 20 µl WB, IP H, M, R, Hm, Mk 42 Rabbit IgG
ATP2A1/SERCA1 (D54G12) Rabbit mAb #12293 20 µl WB H, M R 100 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ATP2A2/SERCA2 (D51B11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C6, and COS-7 cells using PKA C-α (D38C6) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or λ phosphatase-treated, using Phospho-PKA C (Thr197) (D45D3) Rabbit mAb (upper) or PKA C-α Antibody #4782 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 16-month old control (WKY) and spontaneous hypertensive (SHR) rat hearts using Phospho-Phospholamban (Ser16/Thr17) Antibody (left), Phospholamban Antibody #8495 (middle), or GAPDH (14C10) Rabbit mAb #2118 (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from human skeletal muscle and mouse skeletal muscle using ATP2A1/SERCA1 (D54G12) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from mouse heart and 16-month old control (WKY) and spontaneous hypertensive (SHR) rat hearts using Phospholamban (D9W8M) Rabbit mAb.

バックグラウンド

Sarcoplasmic and endoplasmic reticulum Ca2+ ATPases (SERCA) are members of a highly conserved family of Ca2+ pumps (1). ATP2A1 (SERCA1) is a fast-twitch, skeletal muscle sarcoplasmic reticulum (SR) Ca2+ ATPase (2). Multiple ATP2A2 (SERCA2) isoforms have been isolated, with ATP2A2a (SERCA2a) found predominantly in the SR of muscle cells and ATP2A2b (SERCA2b) more ubiquitously expressed in the ER of most cell types (3). Post-translational modification of ATP2A2, including phosphorylation and tyrosine nitration, modify Ca2+ -ATPase activity and calcium transport (4,5).

Phospholamban (PLN) was identified as a major phosphoprotein component of the SR (6). Despite very high expression in cardiac tissue, phospholamban is also expressed in skeletal and smooth muscle (7). Localization of PLN is limited to the SR, where it serves as a regulator of the sarco-endoplasmic reticulum calcium ATPase, SERCA (8). PLN binds directly to SERCA and effectively lowers its affinity for calcium, thus reducing calcium transport into the SR. Phosphorylation of PLN at Ser16 by PKA or myotonic dystrophy protein kinase and/or phosphorylation at Thr17 by Ca2+/calmodulin-dependent protein kinase results in release of PLN from SERCA, relief of this inhibition, and increased calcium uptake by SR (reviewed in 9,10). It has long been held that phosphorylation at Ser16 and Thr17 occurs sequentially, but increasing evidence suggests that phosphorylation, especially at Thr17, may be differentially regulated (reviewed in 11,12).

The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (13). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of the regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. Upon binding of cAMP to the R subunits, the auto-inhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (14). PKA phosphorylation is involved in the regulation of Ca2+ channels, including Cav1.1 in skeletal muscle and Cav1.2 in the heart (reviewed in 15).

使用例
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9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Calcium Ion Regulation Antibody Sampler Kit

Immune Cell Signaling Pathways

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