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#8579 Hippo Signaling Antibody Sampler Kit

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2019年1月17日15時10分 現在
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#8579T1 Kit115,000
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8579 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb #13619 20 µl WB, IP H, M, R 75 Rabbit IgG
LATS1 (C66B5) Rabbit mAb #3477 20 µl WB, IP H, M, Mk 140 Rabbit IgG
Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb #8699 20 µl WB, IHC-P H, M, R, Mk Hm, C, X, Z, B, GP, Hr 24 Rabbit IgG
MOB1 (E1N9D) Rabbit mAb #13730 20 µl WB, IP H, M, R, Hm, Mk 25 Rabbit IgG
MST1 Antibody #3682 20 µl WB, IP H, M, R, Mk, B 59 Rabbit
MST2 Antibody #3952 20 µl WB, IP H, M, R, Mk, B 60 Rabbit
SAV1 (D6M6X) Rabbit mAb #13301 20 µl WB, IP, IF-IC H, M 45 Rabbit IgG
Phospho-YAP (Ser127) (D9W2I) Rabbit mAb #13008 20 µl WB, IP, IHC-P H, M, R 65-75 Rabbit IgG
YAP/TAZ (D24E4) Rabbit mAb #8418 20 µl WB, IP, IHC-P H, M, Mk 50, 70 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster, B=Bovine

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MST1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MST2 Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LATS1 (C66B5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using YAP/TAZ (D24E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, either untreated (-) or treated (+) with H2O2 (2.5 mM, 30 min) and Ramos cells, either untreated (-) or treated (+) with λ phosphatase, using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb (upper) and MOB1 Antibody #3863 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT 116 (higher expressing, left) and ACHN (lower expressing, right) cells using SAV1 (D6M6X) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SAV1 (D6M6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). YAP protein isoform 1 Ser397 corresponds to Ser381 of YAP isoform 2, as reported by Zhao et al. (2010) Genes Dev 24, 72-85 (9).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MOB1 (E1N9D) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells treated with Staurosporine #9953 for the indicated times using MST2 Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using YAP/TAZ (D24E4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of MDA-MB-231 cells, vehicle-treated (-) or treated with Forskolin #3828 (10 μM, 60 min; +) or epinephrine (10 μM, 60 min; +), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Note the induction of YAP (Ser127) phosphorylation after treatment with forskolin or epinephrine, consistent with the findings reported in Xu et al. (2012) [9].


IP

IP

Immunoprecipitation of SAV1 protein from A-204 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SAV1 (D6M6X) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SAV1 (D6M6X) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MDA-MB-231 cells, vehicle treated (-) or treated with epinephrine (10 μM, 60 min; +) or Forskolin #3828 (10 μm, 60 min; +), using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8547 (lower). YAP protein isoform 1 Ser397 corresponds to Ser381 of YAP isoform 2, as reported by Zhao, B. et al. (2010) Genes Dev 24, 72-85 (9).

IP

IP

Immunoprecipitation of MOB1 protein from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MOB1 (E1N9D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MOB1 (E1N9D) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using YAP/TAZ (D24E4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma control (left) or lambda phosphatase-treated (right) using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded COS-7 cell pellets, transfected with YAP (left) or TAZ (right), using YAP/TAZ (D24E4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MCF7 cell pellets, control (left) or H2O2-treated (right), using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma, control (left) or λ-phosphatase treated (right), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb.

Isoform Specificity

Isoform Specificity

Schematic diagram showing annotation of the amino acid sequence surrounding Ser397 in different human YAP isoforms. Asterisk (*) indicates annotation of the phosphorylation site as described in Zhao et al. 2010 [9].

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, A-204 (left) and RL-7 (right), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

バックグラウンド

Hippo signaling is an evolutionarily conserved pathway that controls cell proliferation, apoptosis, and organ size in response to changing cell density levels (1,2). At relative low cell density, transcription co-activators YAP and TAZ bind transcription factors to induce expression of genes that favor cell growth and proliferation. As cell density increases, interaction between membrane-bound upstream hippo pathway regulators trigger activation of cytoplasmic kinases Mst1/2 and LATS1/2. Activated Mst kinase (the eponymous Hippo in Drosophila) associates with the adaptor Sav1 and phosphorylates MOB1 to activate LATS kinase, which phosphorylates YAP and TAZ to suppress cell proliferation (3).

使用例
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Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Hippo Signaling Antibody Sampler Kit

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