#86026 RASSF1 (E7T6O) Rabbit mAb
|RASSF1 (E7T6O) Rabbit mAb recognizes endogenous levels of total RASSF1 protein. The antibody is predicted to detect the following RASSF1 isoforms: 1A, 1D, 1E, 1F, and 1G. It is not predicted to detect isoforms 1B, 1C, or 1H, as these isoforms lack the amino acid sequence corresponding to the antigen.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg50 of human RASSF1 protein.|
ホモロジー (相同性) 検索をご希望の場合 >>>
Western blot analysis of extracts from various cell lines using RASSF1 (E7T6O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Ras association domain-containing protein 1 (RASSF1) is a member of the RASSF protein family (RASSF1-10), scaffold proteins whose members are unified by the presence of a Ras association (RA) domain that gives them structural similarity to Ras effector proteins (1). The RASSF1 gene is located in a genomic region exhibiting loss of heterozygosity in a more than 90% of small cell lung cancers, and up to 50% of non-small cell lung cancers (2), strongly implicating the RASSF1 gene as a tumor suppressor. Moreover, RASSF1 expression in tumor cells has been shown to be frequently suppressed by promoter hypermethylation, further suggesting a tumor suppressor function (3-5). Although multiple isoforms (RASSF1A-H) have been described, generated by alternative splicing and/or promoter usage, RASSF1A and RASSF1C appear to be the most widely expressed in normal tissues, with RASSF1A expression lost most frequently in tumor cells (6). Notably, RASSF1A has been identified as an effector protein in the Hippo signaling pathway, where it promotes association between MST1/2 and LATS1/2, leading to suppressive phosphorylation of the transcriptional co-activators YAP and TAZ (7).
- van der Weyden, L. and Adams, D.J. (2007) Biochim Biophys Acta 1776, 58-85.
- Avruch, J. et al. (2009) J Biol Chem 284, 11001-5.
- Dammann, R. et al. (2001) Cancer Res 61, 3105-9.
- Jiang, D. et al. (2014) Tumour Biol 35, 10449-55.
- Daniunaite, K. et al. (2014) J Urol 192, 1849-55.
- Dammann, R. et al. (2000) Nat Genet 25, 315-9.
- Matallanas, D. et al. (2007) Mol Cell 27, 962-75.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.