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#8652 AMPA Receptor (GluA) Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年3月25日15時35分 現在
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GluA 製品一覧

8652 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb #13185 20 µl WB, IP, IF-F H, M, R Mk, B, Dg 100 Rabbit IgG
AMPA Receptor 2 (GluA2) (D39F2) Rabbit mAb #5306 20 µl WB H, M, R 100 Rabbit IgG
AMPA Receptor 3 (GluA 3) (D47E3) Rabbit mAb #4676 20 µl WB, IP H, M, R 100 Rabbit IgG
AMPA Receptor 4 (GluA 4) (D41A11) XP® Rabbit mAb #8070 20 µl WB, IP, IF-F H, M, R 100 Rabbit IgG
Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) Rabbit mAb #8084 20 µl WB, IP H, M, R 100 Rabbit IgG
Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody #3921 20 µl WB R H, M 100 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human, M=Mouse, R=Rat

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brains using AMPA Receptor 3 (GluA 3) (D47E3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using AMPA Receptor 4 (GluA 4) (D41A11) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and rat brain using Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) Rabbit mAb. The phospho-specificity of the antibody was verified by blocking with a phospho or nonphosphopeptide.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, either 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody (upper) or AMPA Receptor (GluA2/3/4) Antibody #2460 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brains using AMPA Receptor 2 (GluA2) (D39F2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, rat brain, and rat prefrontal cortex tissues using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of rat retina using AMPA Receptor (GluA 4) (D41A11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IP

IP

Immunoprecipitation of AMPA Receptor 1 (GluA1) from mouse brain extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb.


IF-F

IF-F

Confocal immunofluorescent analysis of mouse hippocampus using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).

使用例
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Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

AMPA Receptor (GluA) Antibody Sampler Kit

Immune Cell Signaling Pathways

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