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#8665 Rag and LAMTOR Antibody Sampler Kit

CSTコード 包装
希望納入価格 (円)
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2019年3月25日15時35分 現在
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#8665T1 Kit99,000
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb #8975 20 µl WB, IP, IHC-P, IF-IC H, M, R, Mk 18 Rabbit IgG
LAMTOR2/ROBLD3 (D7C10) Rabbit mAb #8145 20 µl WB, IP H, M, R, Mk 14 Rabbit IgG
LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb #8168 20 µl WB H, M, R, Mk 14 Rabbit IgG
RagA (D8B5) Rabbit mAb #4357 20 µl WB, IP H, M, R, Mk 30 Rabbit IgG
RagC (D31G9) XP® Rabbit mAb #5466 20 µl WB, IP, IHC-P H, M, R, Mk 50 Rabbit IgG
RagB (D18F3) Rabbit mAb #8150 20 µl WB, IP H, Mk 40 Rabbit IgG
RagD Antibody #4470 20 µl WB H 50 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and PANC-1 cells using RagA (D8B5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RagC (D31G9) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LAMTOR2/ROBLD3 (D7C10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RagB (D18F3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RagD Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from mIMCD3, KNRK and COS7 cells using RagA (D8B5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using RagC (D31G9) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RagC (D31G9) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using RagC (D31G9) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The mTORC kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). According to the research literature, RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered to be a viable target for further diagnostic and therapeutic study (5). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit four Rag GTPases to the surface of lysosomes (6).

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Rag and LAMTOR Antibody Sampler Kit

Metabolic Reprogramming in Disease

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