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#8674 Mitochondrial Marker Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#8674T1 Kit107,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
COX IV (3E11) Rabbit mAb #4850 20 µl WB, IP, IHC-P, IF-IC, F H, R, Mk, Z, B, Pg 17 Rabbit IgG
Cytochrome c (136F3) Rabbit mAb #4280 20 µl WB, IHC-P H, M, R, Mk 14 Rabbit IgG
HSP60 (D6F1) XP® Rabbit mAb #12165 20 µl WB, IHC-P, IF-IC, F H, M, R, Hm, Mk, X, Z, B, Pg C, Dg, Hr 60 Rabbit IgG
PHB1 Antibody #2426 20 µl WB, IP H, M, R, Mk 32 Rabbit
Pyruvate Dehydrogenase (C54G1) Rabbit mAb #3205 20 µl WB, IHC-P H, M, R, Mk 43 Rabbit IgG
SDHA (D6J9M) XP® Rabbit mAb  #11998 20 µl WB, IP, IHC-P, IF-IC H, M, R, Hm, Mk 70 Rabbit IgG
SOD1 (71G8) Mouse mAb #4266 20 µl WB, IP H 18 Mouse IgG1
VDAC (D73D12) Rabbit mAb #4661 20 µl WB, IHC-P H, M, R, Mk 32 Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl WB Horse
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, R=Rat, Mk=Monkey, Z=Zebrafish, B=Bovine, Pg=Pig, M=Mouse, Hm=Hamster, X=Xenopus

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of cell lysates from various cell lines, using Cytochrome c (136F3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using PHB1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SOD1 (71G8) Mouse mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using VDAC (D73D12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HSP60 (D6F1) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cytochrome c (136F3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.


IP

IP

Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using HSP60 (D6F1) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using VDAC (D73D12) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor, using Cytochrome c (136F3) Rabbit mAb #4280.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HSP60 (D6F1) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using VDAC (D73D12) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cytochrome c (136F3) Rabbit mAb in the presence of control peptide (left) or Cytochrome c Blocking Peptide #1033 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HSP60 (D6F1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using VDAC (D73D12) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Cytochrome c (136F3) Rabbit mAb .

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using HSP60 (D6F1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using COX IV (3E11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of A-204 cells using HSP60 (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using VDAC (D73D12) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using VDAC (D73D12) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse small intestine using VDAC (D73D12) Rabbit mAb.

バックグラウンド

The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8).

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Mitochondrial Marker Antibody Sampler Kit

Metabolic Reprogramming in Disease

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