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#86770 E-Cadherin (4A2) Mouse mAb (Alexa Fluor® 488 Conjugate)

 

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CSTコード 包装
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2019年1月18日15時10分 現在
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#86770S100 μL63,000
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Cadherin E 製品一覧

感度抗体の由来貯法
内在性Mouse IgG14℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット -
86770 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

免疫蛍光細胞染色 (IF-IC) (1:50)フローサイトメトリー (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのE-Cadherin タンパク質を検出します。他のCadherin タンパク質とは交差しません。
使用抗原
ヒトのE-Cadherin タンパク質に特異的な組換えタンパク質

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社内データ

※下記の社内データは、すべて86770 の推奨プロトコールで実験した結果です。

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 (left) and HeLa (right) cells using E-Cadherin (4A2) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa (blue) and MCF7 (green) cells using E-Cadherin (4A2) Mouse mAb (Alexa Fluor® 488 Conjugate).

バックグラウンド

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

使用例
 
関連製品
14472   E-Cadherin (4A2) Mouse mAb

The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).
DRAQ5 is a registered trademark of Biostatus Limited.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

本製品は試験研究用です。

E-Cadherin (4A2) Mouse mAb (Alexa Fluor® 488 Conjugate)

Immune Cell Signaling Pathways

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