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#8686 Lysine Acetyltransferase Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年3月20日11時40分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
CBP (D6C5) Rabbit mAb #7389 20 µl WB, IP, IF-IC, ChIP, ChIP-seq H, M, R, Mk 300 Rabbit IgG
Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771 20 µl WB, IP, ChIP H, M, Mk R 300 Rabbit
PCAF (C14G9) Rabbit mAb #3378 20 µl WB, IP, ChIP, ChIP-seq H, M, R, Mk B, Hr 93 Rabbit IgG
GCN5L2 (C26A10) Rabbit mAb #3305 20 µl WB, IP, IF-IC H, M, R, Mk B, Hr 94 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431, NIH/3T3, COS and PC12 cells, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GCN5L2 (C26A10) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PCAF (C14G9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CBP (D6C5) Rabbit mAb.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells treated with Forskolin #3828 (30 μM, 1h) and CBP (D6C5) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across NR4A3, a known target gene of CBP (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.


Western Blotting

Western Blotting

Western blot analysis of hypo- or hyper-acetylated recombinant p300 HAT domains, either wild-type or K1499R mutant, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody (upper). Also shown in the corresponding coomassie stained SDS-PAGE gel (lower). (Details are described in Thompson, P.A. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.)

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using GCN5L2 (C26A10) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells treated with Forskolin #3828 (30 µM) and either PCAF (C14G9) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using CBP (D6C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells, treated with Forskolin #3828 (30 μM, 1h) and either CBP (D6C5) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells treated with Forskolin #3828 (30uM) and either Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


バックグラウンド

CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). The role of acetylation of CBP/p300 is of particular interest (2,3). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (4). p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B) (5), and General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) (6) are transcriptional adaptor proteins in addition to HATs. PCAF functions as the catalytic subunit of the PCAF transcriptional co-activator complex (5). GCN5L2 functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (6). PCAF and GCN5L2 acetylate histone H3 at Lys14 and histone H4 at Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (7,8). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD) and general transcription factors (TFIIEβ and TFIIF) (9-12). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (13,14), transcription co-activators (PGC1-α) (15), and nuclear receptors (Steroidogenic Factor 1) (16). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (13-16).

使用例
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Illumina is a registered trademark of Illumina, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Lysine Acetyltransferase Antibody Sampler Kit

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