Confocal immunofluorescent analysis of live HeLa cells using ER-Tracker™ Green (BODIPY® FL Glibenclamide) (green). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
ER-Tracker™ Green (BODIPY® FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY® FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K+ channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.
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