#88378 CHOP (L63F7) Mouse mAb (Alexa Fluor® 488 Conjugate)
|CHOP (L63F7) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total CHOP protein.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CHOP.|
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Flow cytometric analysis of HeLa cells, untreated (blue) or treated with Tunicamycin #12819 (2 μg/ml, 8 hr; green), using CHOP (L63F7) Mouse mAb (Alexa Fluor® 488 Conjugate) (solid lines) or concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (dashed lines).
CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).
|2895 CHOP (L63F7) Mouse mAb|
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