#89241 Neutrophil Elastase (E9C9L) XP® Rabbit mAb
|Neutrophil Elastase (E9C9L) XP® Rabbit mAb recognizes endogenous levels of total neutrophil elastase protein.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human neutrophil elastase protein.|
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Western blot analysis of extracts from various cell lines using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, neutrophil elastase protein expression is not detected in either A-498 or Jurkat cells.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human neutrophil elastase protein (hNE-Myc/DDK; +), using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of neutrophil elastase from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Neutrophil Elastase (E9C9L) XP® Rabbit mAb. Western blot analysis was performed using Neutrophil Elastase (E9C9L) XP® Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.
Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA #4174 (80 nM, 24 hr; left, positive) and A-498 cells (right, negative), using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Flow cytometric analysis of Jurkat cells (blue) and U-937 cells (green) using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Neutrophil elastase is hematopoietic serine protease that belongs to the chymotrypsin superfamily and plays a critical role in the innate immune function of mature neutrophils and monocytes (1,2). Neutrophil elastase is actively synthesized as an inactive zymogen in myelocytic precursor cells of the bone marrow, which then undergoes activation by limited proteolysis and sorting to primary (azurophil) storage granules of mature neutrophil granulocytes for regulated release (3,4). Research studies have shown that neutrophils play a significant role in mediating the inflammatory response through the release of neutrophil elastase, which activates pro-inflammatory cytokines and degrades components of the extracellular matrix and Gram-negative bacteria (5). Mutations in the gene encoding neutrophil elastase, ELA2, have been implicated in hematological diseases such as cyclic and severe congenital neutropenia, which is characterized by defects in promyelocyte maturation (6,7).
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XP is a registered trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.