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#89241 Neutrophil Elastase (E9C9L) XP® Rabbit mAb

 

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2019年3月22日15時35分 現在
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#89241S100 μL61,000
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#89241T20 μL39,000

Neutrophil Elastase 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性30Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
89241 の推奨プロトコール i

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注:各製品に最適化されたプロトコールをリンクしています。

 

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CST推奨プロトコールに欠かせない関連製品

特異性・感度
Neutrophil Elastase (E9C9L) XP® Rabbit mAb recognizes endogenous levels of total neutrophil elastase protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human neutrophil elastase protein.

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社内データ

※下記の社内データは、すべて89241 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, neutrophil elastase protein expression is not detected in either A-498 or Jurkat cells.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human neutrophil elastase protein (hNE-Myc/DDK; +), using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of neutrophil elastase from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Neutrophil Elastase (E9C9L) XP® Rabbit mAb. Western blot analysis was performed using Neutrophil Elastase (E9C9L) XP® Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.


IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA #4174 (80 nM, 24 hr; left, positive) and A-498 cells (right, negative), using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells (blue) and U-937 cells (green) using Neutrophil Elastase (E9C9L) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

バックグラウンド

Neutrophil elastase is hematopoietic serine protease that belongs to the chymotrypsin superfamily and plays a critical role in the innate immune function of mature neutrophils and monocytes (1,2). Neutrophil elastase is actively synthesized as an inactive zymogen in myelocytic precursor cells of the bone marrow, which then undergoes activation by limited proteolysis and sorting to primary (azurophil) storage granules of mature neutrophil granulocytes for regulated release (3,4). Research studies have shown that neutrophils play a significant role in mediating the inflammatory response through the release of neutrophil elastase, which activates pro-inflammatory cytokines and degrades components of the extracellular matrix and Gram-negative bacteria (5). Mutations in the gene encoding neutrophil elastase, ELA2, have been implicated in hematological diseases such as cyclic and severe congenital neutropenia, which is characterized by defects in promyelocyte maturation (6,7).

使用例
 
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13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14569   Myeloperoxidase (E1E7I) XP® Rabbit mAb
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
5127   Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)
6883   SignalFire™ ECL Reagent
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
73778   Protein A Magnetic Beads
7727   Biotinylated Protein Ladder Detection Pack
8457   β-Actin (D6A8) Rabbit mAb
8961   ProLong® Gold Antifade Reagent with DAPI
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Neutrophil Elastase (E9C9L) XP® Rabbit mAb

Metabolic Reprogramming in Disease

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