Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green). Actin filaments were labeled with Alexa Fluor® 555 Phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity (3).
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