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#90111 FnCpf1 (Strain U112) (E7I2B) Rabbit mAb

 

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2019年1月15日15時15分 現在
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Cpf1 製品一覧 | #90111 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
トランスフェクト152Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

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90111 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫蛍光細胞染色 (IF-IC) (1:400)フローサイトメトリー (1:100)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
トランスフェクトレベルのFnCpf (Strain U112) タンパク質を検出します。Cas9 (S. pyogenes)、Cas9 (S. aureus)、AsCpf1 (Strain BV3L6) タンパク質とは交差しません。
使用抗原
Francisella tularensis subsp. novicida Cpf1 (Strain U112) タンパク質のIle841 周辺領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて90111 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing FnCpf1 (Strain U112), AsCpf1 (Strain BV3L6), Cas9 (S. pyogenes), or Cas9 (S. aureus) (+), using FnCpf1 (Strain U112) (E7I2B) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T cells transfected with a construct expressing myc-tagged FnCpf1 (Strain U112) using FnCpf1 (Strain U112) (E7I2B) Rabbit mAb (green) and Myc-Tag (9B11) Mouse mAb #2276 (red). Overlapping signals are shown in yellow. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293 cells, untransfected (blue) or transfected with a construct expressing myc-tagged FnCpf1 (Strain U112) (green), using FnCpf1 (Strain U112) (E7I2B) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


バックグラウンド

CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1 (CRISPR from Prevotella and Francisella) are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1 endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1 utilizes T-Rich protospacer adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1 generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1 bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2, 4).

FnCpf1 (Strain U112) is a Cpf1 enzyme that is derived from Francisella novicida U112 (5).

使用例
 
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The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.
DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

本製品は試験研究用です。

FnCpf1 (Strain U112) (E7I2B) Rabbit mAb

Immune Cell Signaling Pathways

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