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#9092 StemLight™ iPS Cell Reprogramming Antibody Kit

 
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希望納入価格 (円)
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2019年3月20日11時40分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Oct-4A (C30A3) Rabbit mAb #2840 20 µl WB, IF-IC, F H, M 45 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb #3579 20 µl WB, IF-IC, F H B, Dg, Hr 35 Rabbit
Nanog (D73G4) XP® Rabbit mAb #4903 20 µl WB, IHC-P, IF-IC, F H 42 Rabbit IgG
LIN28A (D84C11) XP® Rabbit mAb #3695 20 µl WB, IF-IC, F H R, Mk 26 Rabbit IgG
KLF4 Antibody #4038 20 µl WB H, M, Mk 65 Rabbit
c-Myc (D84C12) Rabbit mAb #5605 20 µl WB, IF-IC, F H, M, R Dg, Pg 57-65 Rabbit IgG

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat

貯法
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社内データ

IF-IC

IF-IC

Confocal immunofluorescent analysis of iPS cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, Nanog (D73G4) XP® Rabbit mAb #4903, c-Myc (D84C12) XP® Rabbit mAb #5605, and LIN28A (D84C11) XP® Rabbit mAb #3695 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of lentiviral transduced human fibroblast cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, c-Myc (D84C12) XP® Rabbit mAb #5605, and KLF4 Antibody #4038 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and mouse embryonic stem cells (mESCs) using Oct-4A (C30A3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts of NTERA-2 and IGROV1 cells using LIN28A (D84C11) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KLF4 antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of HEK293 cell extracts, untreated (-) or c-Myc knock-out (+), using c-Myc (D84C12) Rabbit mAb Antibody #5605 (upper) or β-actin (13E5) Rabbit mAb #4970 (lower).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C30A3) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using LIN28A (D84C11) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® c-Myc siRNA I #6341, using c-Myc (D84C12) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 (left) and mouse embryonic stem (mES) cells growing on mouse embryonic fibroblast (MEF) feeder cells (right) using Oct-4A (C30A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells using Lin28A (D84C11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using c-Myc (D84C12) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raji cells using c-Myc (D84C12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red).

バックグラウンド

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells.

Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated.

SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neuraminadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください

DRAQ5 is a registered trademark of Biostatus Limited.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

StemLight™ iPS Cell Reprogramming Antibody Kit

Immune Cell Signaling Pathways

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