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#9092 StemLight™ iPS Cell Reprogramming Antibody Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Oct-4A (C30A3) Rabbit mAb #2840 20 µl WB, IF-IC, F H, M 45 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb #3579 20 µl WB, IF-IC, F H B, Dg, Hr 35 Rabbit
Nanog (D73G4) XP® Rabbit mAb #4903 20 µl WB, IHC-P, IF-IC, F H 42 Rabbit IgG
LIN28A (D84C11) XP® Rabbit mAb #3695 20 µl WB, IF-IC, F H R, Mk 26 Rabbit IgG
KLF4 Antibody #4038 20 µl WB H, M, Mk 65 Rabbit
c-Myc (D84C12) Rabbit mAb #5605 20 µl WB, IF-IC, F H, M, R Dg, Pg 57-65 Rabbit IgG

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat

貯法
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社内データ

IF-IC

IF-IC

Confocal immunofluorescent analysis of iPS cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, Nanog (D73G4) XP® Rabbit mAb #4903, c-Myc (D84C12) XP® Rabbit mAb #5605, and LIN28A (D84C11) XP® Rabbit mAb #3695 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of lentiviral transduced human fibroblast cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, c-Myc (D84C12) XP® Rabbit mAb #5605, and KLF4 Antibody #4038 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and mouse embryonic stem cells (mESCs) using Oct-4A (C30A3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts of NTERA-2 and IGROV1 cells using LIN28A (D84C11) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KLF4 antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® c-Myc siRNA I #6341, using c-Myc (D84C12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C30A3) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using LIN28A (D84C11) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using c-Myc (D84C12) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 (left) and mouse embryonic stem (mES) cells growing on mouse embryonic fibroblast (MEF) feeder cells (right) using Oct-4A (C30A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells using Lin28A (D84C11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raji cells using c-Myc (D84C12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


バックグラウンド

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells.

Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated.

SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neuraminadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください

DRAQ5 is a registered trademark of Biostatus Limited.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

StemLight™ iPS Cell Reprogramming Antibody Kit

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