CST logo

#9093 StemLight™ Pluripotency Transcription Factor Antibody Kit

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年1月17日15時10分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#9093T1 Kit61,000
ログインすると国内在庫状況がご確認いただけます。

Stem Cell Kit 製品一覧

9093 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Nanog (D73G4) XP® Rabbit mAb #4903 20 µl WB, IHC-P, IF-IC, F H 42 Rabbit IgG
Oct-4A (C30A3) Rabbit mAb #2840 20 µl WB, IF-IC, F H, M 45 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb #3579 20 µl WB, IF-IC, F H B, Dg, Hr 35 Rabbit

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse

貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and mouse embryonic stem cells (mESCs) using Oct-4A (C30A3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C30A3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 (left) and mouse embryonic stem (mES) cells growing on mouse embryonic fibroblast (MEF) feeder cells (right) using Oct-4A (C30A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

バックグラウンド

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma, and induced pluripotent cells.

Oct-4, Sox2, and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2, along with KLF4 and c-Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcriptional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog, and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog, and Sox2 is downregulated.

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください

DRAQ5 is a registered trademark of Biostatus Limited.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

StemLight™ Pluripotency Transcription Factor Antibody Kit

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications