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#9094 StemLight™ Pluripotency Surface Marker Antibody Kit

 
CSTコード 包装
希望納入価格 (円)
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2019年3月25日15時35分 現在
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#9094T1 Kit61,000
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
SSEA4 (MC813) Mouse mAb #4755 20 µl IF-IC, F H N/A Mouse IgG3
TRA-1-60(S) (TRA-1-60(S)) Mouse mAb #4746 20 µl WB, IHC-P, IF-IC, F H 200-400 Mouse IgM
TRA-1-81 (TRA-1-81) Mouse mAb #4745 20 µl IHC-P, IF-IC, F H 200-400 Mouse IgM

Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human

貯法
-20℃
社内データ

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells, using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb, TRA-1-81 (TRA-1-81) Mouse mAb, and SSEA4 (MC813) Mouse mAb. Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-81 (TRA-1-81) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA cells (green) using SSEA4 (MC813) Mouse mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NCCIT cells (green) using TRA-1-81 (TRA-1-81) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 cells using SSEA4 (MC813) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells using TRA-1-81 (TRA-1-81) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


バックグラウンド

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm, and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma, and induced pluripotent cells.

SSEA4, TRA-1-81, and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (1). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (2). These epitopes are neuraminidase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (3).

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

StemLight™ Pluripotency Surface Marker Antibody Kit

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