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#92964 ARID1B/BAF250B (E9J4T) Rabbit mAb

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2019年3月22日15時35分 現在
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#92964S100 μL56,000
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ARID1B 製品一覧 | #92964 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性250, 280Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス -
92964 の推奨プロトコール i

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特異性・感度
内在性レベルのARID1B/BAF250B タンパク質を検出します。 ARID1A/BAF250A タンパク質とは交差しません。
使用抗原
ヒトのARID1B/BAF250B タンパク質のAla1320 周辺領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて92964 の推奨プロトコールで実験した結果です。

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free/charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 min and ARID1B/BAF250B (E9J4T) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across TFF1/pS2, a known target gene of ARID1B (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ARID1B/BAF250B (E9J4T) Rabbit mAb (upper) and α-Actinin (D6F6) Rabbit mAb #6487 (lower). MIA PaCa-2 cells do not express ARID1B/BAF250B protein.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free/charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 min and either ARID1B/BAF250B (E9J4T) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human pS2 Promoter Primers #9702, SimpleChIP® Human ESR1 Promoter Primers #9673, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


バックグラウンド

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

ARID1B (A-T rich interacting domain 1B), also known as BAF250B, is a DNA-binding member of the SWI/SNF complex. It has 60% homology with ARID1A/BAF250A, and the proteins are mutually exclusive members of the complex, akin to Brg1 and BRM (10). ARID1B plays a role in synapse formation and dendritic arborization in neuronal development, and haploinsufficiency of ARID1B has been reported in intellectual disability (11-13). Mutations in ARID1B have also been shown in Coffin-Siris syndrome (14). ARID1B/BAF250B is a critical vulnerability in ARID1A/BAF250A mutant cancers, and could be explored as a potential therapeutic target (15).

使用例
 
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Illumina is a registered trademark of Illumina, Inc.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

ARID1B/BAF250B (E9J4T) Rabbit mAb

Metabolic Reprogramming in Disease

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