#9320 Src Family Antibody Sampler Kit
|Src (32G6) Rabbit mAb #2123||20 µl||WB, IP||H, M, R, Mk||C||60||Rabbit IgG|
|Csk (C74C1) Rabbit mAb #4980||20 µl||WB||H, M, R, Mk, Pg||50||Rabbit IgG|
|Fyn Antibody #4023||20 µl||WB||H, M||59||Rabbit|
|Lck (D88) XP® Rabbit mAb #2984||20 µl||WB, IHC-P||H||56||Rabbit IgG|
|Lyn (C13F9) Rabbit mAb #2796||20 µl||WB, IP, IHC-P||H, M, R, Mk||56||Rabbit IgG|
|Yes Antibody #3201||20 µl||WB, IP||H, Mk, B||60||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||WB||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Pg=Pig, B=Bovine
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of cell extracts from various cell lines, using Fyn Antibody.
Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb.
Western blot analysis of cell extracts from various cell types using Lyn (C13F9) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Csk (C74C1) Rabbit mAb.
Western blot analysis of extracts from Ramos and Jurkat cells using LcK (D88) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded non-hodgkins lymphoma using Lyn (C13F9) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Lck (D88) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lyn (C13F9) Rabbit mAb in the presence of control peptide (left) and antigen specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human lymphoma using Lck (D88) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Colo205 (positive) (left) and HeLa (negative) (right) cell pellets using Lck (D88) XP® Rabbit mAb.
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
The kinase Fyn plays a role in T-cell receptor signaling and also helps in memory formation and adhesion mediated signaling (3,4). Lymphocyte specific kinase Lck is essential in the differentiation and activation of T-cells (3). Hematopoietic Lyn kinase, also phosphorylated by Csk, is involved in the regulation of B-cell function, migration and development (5). The ubiquitously expressed kinase Yes acts downstream of several different cell surface receptors, including G-protein-coupled receptors, and is involved in the regulation of angiogenesis, the cell cycle and cell adhesion (6,7).
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- Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
- Isosaka, T. et al. (2008) Eur. J. Neurosci. 28, 973-981.
- Seo, S. et al. (2001) J. Immunol. 166, 3710-3716.
- Summy, J.M. et al. (2003) J. Cell Sci. 116, 2585-2598.
- Werdich, X.Q. and Penn, J.S. (2005) Angiogenesis 8, 315-326.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.