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#9320 Src Family Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#9320T1 Kit92,000
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Src 製品一覧

9320 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Src (32G6) Rabbit mAb #2123 20 µl WB, IP H, M, R, Mk C 60 Rabbit IgG
Csk (C74C1) Rabbit mAb #4980 20 µl WB H, M, R, Mk, Pg 50 Rabbit IgG
Fyn Antibody #4023 20 µl WB H, M 59 Rabbit
Lck (D88) XP® Rabbit mAb #2984 20 µl WB, IHC-P H 56 Rabbit IgG
Lyn (C13F9) Rabbit mAb #2796 20 µl WB, IP, IHC-P H, M, R, Mk 56 Rabbit IgG
Yes Antibody #3201 20 µl WB, IP H, Mk, B 60 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Pg=Pig, B=Bovine

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Fyn Antibody.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell types using Lyn (C13F9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Csk (C74C1) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos and Jurkat cells using LcK (D88) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Yes Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded non-hodgkins lymphoma using Lyn (C13F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Lck (D88) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lyn (C13F9) Rabbit mAb in the presence of control peptide (left) and antigen specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using Lck (D88) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Colo205 (positive) (left) and HeLa (negative) (right) cell pellets using Lck (D88) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Lck (D88) XP® Rabbit mAb.

バックグラウンド

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

The kinase Fyn plays a role in T-cell receptor signaling and also helps in memory formation and adhesion mediated signaling (3,4). Lymphocyte specific kinase Lck is essential in the differentiation and activation of T-cells (3). Hematopoietic Lyn kinase, also phosphorylated by Csk, is involved in the regulation of B-cell function, migration and development (5). The ubiquitously expressed kinase Yes acts downstream of several different cell surface receptors, including G-protein-coupled receptors, and is involved in the regulation of angiogenesis, the cell cycle and cell adhesion (6,7).

使用例
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関連製品
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12757   SignalFire™ Elite ECL Reagent
2101   Phospho-Src Family (Tyr416) Antibody
2102   Non-phospho-Src (Tyr416) (7G9) Mouse mAb
2105   Phospho-Src (Tyr527) Antibody
2107   Non-phospho-Src (Tyr527) Antibody
2108   Src Antibody
2109   Src (36D10) Rabbit mAb
2110   Src (L4A1) Mouse mAb
2731   Phospho-Lyn (Tyr507) Antibody
2732   Lyn Antibody
2751   Phospho-Lck (Tyr505) Antibody
2755   Fgr Antibody
4576   Lyn (5G2) Mouse mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9935   Src Antibody Sampler Kit

XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Src Family Antibody Sampler Kit

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