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#9326 p90RSK Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#9326T1 Kit75,000
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9326 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb #8753 20 µl WB, IP, IHC-P, IF-IC H, Mk C, X, Dg, Hr 90 Rabbit IgG
Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb #11989 20 µl WB, IHC-P, IF-IC H, M, R, Mk, Mi C, X, Z, B, Dg, Pg, Hr 90 Rabbit IgG
Phospho-p90RSK (Thr573) Antibody #9346 20 µl WB, IF-IC H, M, R Hm, C, X, Z 90 Rabbit
RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 20 µl WB, IP, IF-IC H, M, R, Mk, GP 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, Mk=Monkey, M=Mouse, R=Rat, Mi=Mink, GP=Guinea Pig

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, L929 and AR42J cells, using RSK1/RSK2/RSK3 (32D7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr573) Antibody (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, COS-7, and A-431 cells, starved overnight and either untreated (-) or treated (+) with TPA #4174 (200 nM, 15 min) or Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, starved overnight and either untreated (-) or treated (+) with TPA #4174 (200 nM, 15 min), Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min), or Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912 (100 ng/mL, 15 min) as indicated, using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with RSK1/RSK2/RSK3 (32D7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TPA-treated (right), using Phospho-p90RSK (Thr573) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

IP

IP

Immunoprecipitation of phospho-p90RSK (Thr359) from extracts of HeLa cells, serum starved overnight and either untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Normal Rabbit IgG #2729 (lanes 5 and 6) or Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (lanes 3 and 4). Lanes 1 and 2 are 10% input. Western blot analysis was performed using RSK1/RSK2/RSK3 Antibody #9347.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A-431 cell pellets, untreated (left) or treated with hEGF #8916 (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with TPA #4174 (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded colon carcinoma using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells, serum-starved (left), treated with TPA #4174 (200 nM, 15 min; center), or treated with TPA followed by λ phosphatase (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-IC

IF-IC

Confocal immunofluorescent analysis of A-431 cells, serum-starved (left), treated with hEGF #8916 (100 ng/mL, 15 min; center), or treated with hEGF and λ phosphatase (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

使用例
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9341   Phospho-p90RSK (Ser380) Antibody
9343   RSK3 Antibody
9344   Phospho-p90RSK (Thr359/Ser363) Antibody
9346   Phospho-p90RSK (Thr573) Antibody
9347   RSK1/RSK2/RSK3 Antibody
9348   Phospho-RSK3 (Thr356/Ser360) Antibody
9355   RSK1/RSK2/RSK3 (32D7) Rabbit mAb

DRAQ5 is a registered trademark of Biostatus Limited.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

p90RSK Antibody Sampler Kit

Immune Cell Signaling Pathways

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製品特集

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