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#9328 c-Oncogene Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9328T1 Kit115,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
c-Fos Antibody #4384 20 µl WB H, M, R 62 Rabbit
c-Abl Antibody #2862 20 µl WB, IP H, M, R 135 (c-Abl); 210 (Bcr-Abl) Rabbit
c-Jun (60A8) Rabbit mAb #9165 20 µl WB, IP, IHC-P, IF-IC, F, ChIP, ChIP-seq H, M, R, Mk 43, 48 Rabbit IgG
c-Kit (D13A2) XP® Rabbit mAb #3074 20 µl WB, IP, IF-IC H, M 120 and 145 Rabbit
c-Myc (D84C12) Rabbit mAb #5605 20 µl WB, IF-IC, F H, M, R Dg, Pg 57-65 Rabbit IgG
c-Raf Antibody #9422 20 µl WB H, M, R, Mk 65 to 75 Rabbit
Ras (27H5) Rabbit mAb #3339 20 µl WB H, M, R, Mk, Dm 21 Rabbit IgG
c-Rel Antibody #4727 20 µl WB, IP, IHC-P, IF-IC, F H, Mk 78 Rabbit
Src (36D10) Rabbit mAb #2109 20 µl WB, IP, IHC-P, IF-F, IF-IC, F H, M, R, Hm, Mk, B, Pg C 60 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq, IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Dm=D. melanogaster, Hm=Hamster, B=Bovine, Pg=Pig

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using c-Abl Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells, untreated or UV-treated, using c-Jun (60A8) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C2C12 or PC12 cells, untreated or TPA-treated (200 nM for 30 minutes), using c-Raf Antibody #9422.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Src (36D10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, RAW, and H-4-IIE cells serum-starved overnight and TPA-stimulated for 4 hours, using c-Fos Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from K562, Raji and 293 cells, using c-Rel Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T, C2C12 and C6 cells using Ras (27H5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H526 cells using c-Kit (D13A2) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® c-Myc siRNA I #6341, using c-Myc (D84C12) Rabbit mAb.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and c-Jun (60A8) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across Dclk1, a known target gene of c-Jun (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human astrocytoma, using c-Jun (60A8) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Src (36D10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using c-Rel Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using c-Myc (D84C12) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of NCI-H526 (left) and Jurkat (right) cells using c-Kit (D13A2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either of c-Jun (60A8) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using c-Jun (60A8) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma using Src (36D10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Hodgkin's lymphoma, showing nuclear and cytoplasmic localization using c-Rel Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raji cells using c-Myc (D84C12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using c-Jun (60A8) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Src (36D10) Rabbit mAb in the presence of control peptide (left) or Src Blocking Peptide #1235 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human skin, using c-Rel Antibody.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells using c-Rel Antibody (blue) compared to a nonspecific negative control antibody (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A-431 cells using Src (36D10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using c-Jun (60A8) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, using c-Jun (60A8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TNFα-treated (#2169, 20 ng/ml for 20 min, right), using c-Rel Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).

IF-F

IF-F

Confocal immunofluorescent analysis of rat colon labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor=DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The regulation of cell growth, differentiation and programmed death is coordinated by several sets of proteins that comprise essential signal transduction pathways. Many of these key regulatory proteins are encoded by proto-oncogenes, which can be activated (altered) to change the typical cell program to one of abnormal cell growth and unregulated development. Proteins encoded by proto-oncogenes include growth factors and other ligands, receptor proteins, tyrosine kinases, various regulatory proteins (i.e. GTPases) and transcription factors. Together these proteins comprise the basic elements of cell signaling pathways; altered expression or mutation of one or more of these components can lead to oncogenic growth (reviewed in 1).

Non-receptor (i.e. cytoplasmic, nuclear) tyrosine kinases such as c-Abl and Src play key roles in the regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (2,3). Alteration of the corresponding c-Abl and Src proto-oncogenes is associated with oncogenesis; Abl1-BCR gene translocations result in chronic myelogenous leukemia (CML) while constitutively active Src is seen in some patients with colon cancer and altered Src expression is seen in a wide array of cancers (2,4). Regulation of Raf tyrosine kinase by Ras GTPase controls downstream kinases in the MEK/MAPK signaling pathway (5). Activation of the Ras and Raf proto-oncogenes are common in human cancers and both proteins are seen as potential therapeutic targets (6). The receptor tyrosine kinase c-Kit plays a critical role in activation and growth of hematopoietic stem cells (7); mutations that inhibit c-Kit kinase activity are associated with a variety of developmental disorders while mutations producing constitutively active c-Kit can result in mastocytosis and gastrointestinal stromal tumors (8). The alteration of key transcription factors such as c-Fos, c-Jun, c-Myc and c-Rel that are normally responsible for regulating cell and tissue growth, differentiation and the inflammation/immune response, can also result in unregulated, oncogenic cell growth (9-12).

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Illumina is a registered trademark of Illumina, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

c-Oncogene Antibody Sampler Kit

Immune Cell Signaling Pathways

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