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#9330 FAK Antibody Sampler Kit

CSTコード 包装
希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9330T1 Kit75,000
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FAK 製品一覧

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-FAK (Tyr576/577) Antibody #3281 20 µl WB, IP H, M, R C, X 125 Rabbit
Phospho-FAK (Tyr925) Antibody #3284 20 µl WB, IP H, M M, R, C 125 Rabbit
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556 20 µl WB, IP H M, R, Mk, B 125 Rabbit IgG
FAK (D2R2E) Rabbit mAb #13009 20 µl WB, IP H, M 125 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or anti-CD3 antibody-treated (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr925) Antibody (upper) or FAK antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody (upper) or FAK antibody (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FAK (D2R2E) Rabbit mAb.

IP

IP

Immunoprecipitation of FAK from U-87 MG cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FAK (D2R2E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FAK (D2R2E) Rabbit mAb.


バックグラウンド

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

FAK Antibody Sampler Kit

Immune Cell Signaling Pathways

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