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#9370 c-Kit Antibody Sampler Kit

CSTコード 包装
希望納入価格 (円)
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2019年1月17日15時10分 現在
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Kit 製品一覧

9370 の推奨プロトコール i

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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb #3073 20 µl WB, IP H 145 Rabbit IgG
Phospho-c-Kit (Tyr719) Antibody #3391 20 µl WB H, M 120, 145 Rabbit
c-Kit (D13A2) XP® Rabbit mAb #3074 20 µl WB, IP, IF-IC H, M 120 and 145 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from MO7e cells, untreated or stimulated with SCF #9907 (50 ng/ml for 2 minutes), using Phospho-c-Kit (Tyr719) Antibody (upper) or c-Kit Antibody #3392 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H526 cells using c-Kit (D13A2) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts of H526 cells, untreated or treated with SCF, using Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb (upper) or c-Kit Antibody #3392 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCI-H526 (left) and Jurkat (right) cells using c-Kit (D13A2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
3308   c-Kit (Ab81) Mouse mAb
3310   c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate)
3392   c-Kit Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

c-Kit Antibody Sampler Kit

Immune Cell Signaling Pathways

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