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#94853 Human Interleukin-8 (hIL-8)

  • Human Interleukin-8 (hIL-8) Data Sheet PDF

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CSTコード 包装
希望納入価格 (円)
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2019年1月21日15時15分 現在
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IL-8 製品一覧

由来
ヒトのInterleukin-8 (hIL-8) を大腸菌発現系を用いて発現、精製したリコンビナントタンパク質。
※この製品は凍結乾燥品で、BSA などのキャリアタンパク質が添加されていません。
純度
95%以上
貯法
-20℃
社内データ

Coomassie Gel

Coomassie Gel

The purity of recombinant hIL-8 was determined by SDS-PAGE of 1.5 µg reduced (+) and non-reduced (-) recombinant hIL-8 and staining overnight with Coomassie Blue.

Bioactivity

Bioactivity

A cell migration assay was used to measure the effect of hIL-8 on human neutrophil cells. The neutrophils were isolated and loaded into an upper chamber over a permeable membrane at a concentration of 2x106 cells/mL. The hIL-8 was loaded into the bottom chamber at a concentration of 10 ng/mL to induce cell migration. PBS was used as a vehicle control. The chambers were incubated for an hour at 37oC and 5% CO2 prior to being collected and counted on a hemocytometer to measure the percentage of cell migration.

バックグラウンド

The prototypical CXC chemokine, IL-8, is best known for effects on neutrophils, specifically its ability to act as a chemoattractant and activate degranulation and respiratory burst (1,2,3).

IL-8 is produced by a number of cell types, including monocytes, T cells, neutrophils, fibroblasts, endothelial cells, and others (1). In addition to its effects on neutrophils, IL-8 promotes angiogenesis, inhibits endothelial cell apoptosis, and promotes the proliferation of melanoma cells in an autocrine fashion (1). As a result, IL-8 may be a contributing factor to the development of certain cancers (1,2). There are two distinct receptors for IL-8, CXCR1 and CXCR2; both are G protein-coupled receptors (1). Ligand binding induces Ca2+ mobilization and activates the PI3K, PKC, Rho, and Rac pathways (1,3).

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Human Interleukin-8 (hIL-8)

Metabolic Reprogramming in Disease

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