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#94905 PhosphoPlus® Glycogen Synthase (Ser641) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
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2019年1月21日15時15分 現在
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#94905S1 Kit113,000
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94905 の推奨プロトコール i

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キット内容 容量 用途 種交差性 Mol. Wt. アイソタイプ
Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb #47043 100 µl WB, IP, IHC-P H, M, R, Mk 85-90 kDa Rabbit IgG
Glycogen Synthase (15B1) Rabbit mAb #3886 100 µl WB, IP, IHC-P H, M, R 84 kDa Rabbit IgG

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)

Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Glycogen Synthase (15B1) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated (+) with λ phosphatase and calf intestinal alkaline phosphatase (CIP), using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (upper) or Glycogen Synthase (15B1) Rabbit mAb #3886 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Glycogen Synthase (15B1) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma, untreated (left) or λ phosphatase treated (right), using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb.

バックグラウンド

Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals -- muscle isoform (GYS1) and liver isoform (GYS2) respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649 and Ser653) inhibiting its activity (2, 3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
3886   Glycogen Synthase (15B1) Rabbit mAb
3891   Phospho-Glycogen Synthase (Ser641) Antibody
3893   Glycogen Synthase Antibody
47043   Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9331   Phospho-GSK-3α/β (Ser21/9) Antibody
9336   Phospho-GSK-3β (Ser9) Antibody
9337   Phospho-GSK-3α (Ser21) (46H12) Mouse mAb
9338   GSK-3α Antibody
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
CST is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

PhosphoPlus® Glycogen Synthase (Ser641) Antibody Duet

Immune Cell Signaling Pathways

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