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#9555 cdc25C Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9555T1 Kit61,000
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CDC25C 製品一覧

9555 の推奨プロトコール i

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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
Phospho-cdc25C (Thr48) Antibody #9527 20 µl WB H 75 Rabbit
Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 20 µl WB, IP, IHC-P H, Mk 60 Rabbit IgG
cdc25C (5H9) Rabbit mAb #4688 20 µl WB H 60, 75 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, Mk=Monkey

貯法
-20℃
社内データ

Western blot analysis of extracts from HeLa, 293 and K-562 cells using cdc25C (5H9) Rabbit mAb #4688.

Western blot analysis of extracts from HT-29 cells, untreated, nocodazole-treated or λ phosphatase-treated, using Phospho-cdc25C (Ser216) Rabbit mAb #4901 (upper) and cdc25C (5H9) Rabbit mAb #4688 (lower).

Western blot analysis of extracts from HT-29 cells, untreated, nocodazole-treated or λ phosphatase-treated, using Phospho-cdc25C (Thr48) #9527 (upper) and cdc25C (5H9) Rabbit mAb #4688 (lower).


Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, 293 and K562 cells, using cdc25C (5H9) Rabbit mAb.

Western Blotting

Western Blotting

Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit Mab, #4688, (lower).


Western Blotting

Western Blotting

Western Blot analysis of HT29 cell extracts untreated, nocodazole-treated and lambda phosphotase-treated using Phospho-cdc25C (Thr48) (upper), and cdc25C (5H9) Rabbit mAb, #4688 (lower).

Western Blotting

Western Blotting

Western blot analysis of GST-cdc25C, unphosphorylated or phosphorylated in vitro by Chk2, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb (upper) or cdc25C antibody #9522 (lower).

Western Blotting

Western Blotting

Western blot analysis of HT29 cell extracts untreated, nocodazole-treated, and λ phosphotase-treated using Phospho-cdc25C (Thr48), #9527 (upper), and cdc25C (5H9) Rabbit mAb (lower).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide #1190 (right).

バックグラウンド

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

Full activation of cdc25C involves phosphorylation at more than 12 different sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization on phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10).

使用例
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2348   Phospho-Chk1 (Ser345) (133D3) Rabbit mAb
2661   Phospho-Chk2 (Thr68) Antibody
3652   cdc25A Antibody
4688   cdc25C (5H9) Rabbit mAb
4901   Phospho-cdc25C (Ser216) (63F9) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9111   Phospho-cdc2 (Tyr15) Antibody
9525   cdc25B Antibody
9527   Phospho-cdc25C (Thr48) Antibody
9528   Phospho-cdc25C (Ser216) Antibody

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

cdc25C Antibody Sampler Kit

Immune Cell Signaling Pathways

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製品特集

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