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#9615 Phospho-(Ser) Kinase Substrate Antibody Sampler Kit

 
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2019年3月20日11時40分 現在
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#9615T1 Kit75,000
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Kinase substrate 製品一覧

9615 の推奨プロトコール i

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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 20 µl WB, IP, IHC-P, E-P H, All Rabbit IgG
Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981 20 µl WB, IP, E-P H, M, All Rabbit
Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 20 µl WB, IP, E-P H, All Mouse IgG1
Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix #9477 20 µl WB, IP, E-P H, All Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl WB Horse

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA)
Reactivity Key: H=Human, All=All Species Expected, M=Mouse

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb (left) or Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A treated, using Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A431 cells, untreated or treated in culture with EGF, and HeLa cells, untreated, hydroxyurea-treated (G1/S) or Nocodazole-treated (G2/M), using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using

Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

バックグラウンド

Phospho-(Ser) kinases and phosphatases play critical roles in a wide range of biological processes. Each phospho-(Ser) kinase phosphorylates serine within a specific motif. The MAPK and CDK families of serine protein kinases phosphorylate serine followed by proline residue (1-3). The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids and S* is the phosphorylation site (4-6). MAPK phosphorylates substrates with the concensus sequence PX(S*)P. The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (7-11). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (12). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (12). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FoxO3A, PKC and c-Raf (11, 13). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCγ, IRS-1 and BCR (12). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. Antibodies specific to particular kinase substrates are invaluable reagents in determining kinase activity and identifying potential new kinase substrates. CST has developed antibodies that recognize phosphorylated serine within the context of a protein motif that is phosphorylated by MAPK/CDK, CDKs or 14-3-3. As shown by DELFIA or ELISA, each phospho-(Ser) kinase substrate antibody in this sampler kit is specific to its kinase substrate motif.

使用例
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9441   Acetylated-Lysine Antibody
9601   Phospho-(Ser) 14-3-3 Binding Motif Antibody
9614   Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb
9624   Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
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9634   Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb
9691   Nitro-Tyrosine Antibody
9920   Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.
MultiMab is a trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Phospho-(Ser) Kinase Substrate Antibody Sampler Kit

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