CST logo

#96397 FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific)

 

10th Anniversary大感謝祭:一次抗体50% OFF対象製品

CSTコード 包装
希望納入価格 (円)
キャンペーン価格 (円)
国内在庫 i
2018年11月15日15時25分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#96397S100 μL58,00029,000
ログインすると国内在庫状況がご確認いただけます。
#96397T20 μL39,00019,500

FcgR2B 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性60-80Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

マウス -
96397 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫組織染色 (パラフィン) (1:100)フローサイトメトリー (1:400)Immunohistochemistry (Leica® Bond™) (1:100)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのFcγRIIB タンパク質を検出します。
使用抗原
マウスのFcγRIIB タンパク質のPro284 周辺領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて96397 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of FcγRIIB from J774A.1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific). Western blot analysis was performed using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using Fc-gamma-RIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) performed on the Leica® BOND™ Rx.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse forestomach using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded J774A.1 cell pellet (left, positive) or NIH/3T3 cell pellet (right, negative) using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse placenta using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of mouse splenocytes using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) (right) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Samples were co-stained with CD45R (B220)-APC to distinguish the B cell population. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Total viable cells were used for analysis.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of mouse splenocytes using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) (right) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Samples were co-stained with CD3-FITC to distinguish the T cell population. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. Total viable cells were used for analysis.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells (blue) and J774A.1 cells (green) using FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

バックグラウンド

FcγRIIB (CD32B) is a low affinity, IgG Fc-binding receptor expressed on B cells, monocytes, macrophages, and dendritic cells (DCs) (1-3). It is the inhibitory Fc receptor and signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) within its carboxy-terminal cytoplasmic tail (2). Binding of immune complexes to FcγRIIB results in tyrosine phosphorylation of the ITIM motif at Tyr292 and recruitment of the phosphatase SHIP, which mediates inhibitory effects on immune cell activation (2,4). In this way, FcγRIIB suppresses the effects of activating Fc-binding receptors (3). For example, mice deficient for FcγRIIB have greater T cell and DC responses following injection of immune complexes (5, 6). In addition, FcγRIIB plays a role in B cell affinity maturation (7). Signaling through FcγRIIB in the absence of signaling through the B cell receptor (BCR) is proapoptotic, while signaling through FcγRIIB and the BCR simultaneously attenuates the apoptotic signal and results in selection of B cells with higher antigen affinity (7).

使用例
 
関連製品
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
Tween is a registered trademark of ICI Americas, Inc.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

FcγRIIB (D8F9C) XP® Rabbit mAb (Mouse Specific)

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications