CST logo

#96443 PhosphoPlus® Sox2 (Ser250/Ser251) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年3月22日15時35分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#96443S1 Kit113,000
ログインすると国内在庫状況がご確認いただけます。

Sox2 製品一覧

96443 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 ホモロジー† Mol. Wt. アイソタイプ
Phospho-Sox2 (Ser250/Ser251) (A2I7G) Rabbit mAb #92186 100 µl WB, IP H, M Rat 35 kDa Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb #3579 100 µl WB, IF-IC, F H Bovine, Dog, Horse 35 kDa Rabbit

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Reactivity Key: H=Human, M=Mouse

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT cells either untreated (-) or treated with lambda phosphatase (+) using Phospho-Sox2 (Ser250/Ser251) (A2I7G) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

バックグラウンド

Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal (1). Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state (2,3). Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development (4,5). siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (6). Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state (7,8). Additional evidence demonstrates that Sox2 is also present in adult multipotent progenitors that give rise to some adult epithelial tissues, including several glands, the glandular stomach, testes, and cervix. Sox2 is thought to regulate target gene expression important for survival and regeneration of these tissues (9).

Phosphorylation on these and other sites on Sox2 have been observed in pluripotent cells as they undergo differentiation, although the mechanism and consequence of this potential regulation is not clear (10).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

PhosphoPlus® Sox2 (Ser250/Ser251) Antibody Duet

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications