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#9655 PI3 Kinase Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody #4228 20 µl WB, IP M 60 and 85 Rabbit
PI3 Kinase p85 (19H8) Rabbit mAb #4257 20 µl WB, IP H, M, R C 85 Rabbit IgG
PI3 Kinase p110α (C73F8) Rabbit mAb #4249 20 µl WB, IP H, M, R, B 110 Rabbit IgG
PI3 Kinase p110β (C33D4) Rabbit mAb #3011 20 µl WB, IP H 110 Rabbit IgG
PI3 Kinase Class III (D4E2) Rabbit mAb #3358 20 µl WB H, M, R, Mk 100 Rabbit IgG
PI3 Kinase p110γ (D55D5) Rabbit mAb #5405 20 µl WB, IP H, M R 110 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: M=Mouse, H=Human, R=Rat, B=Bovine, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3-Src cells, untreated or treated with lambda phosphatase and from C2C12 cells, untreated or treated with H2O2, using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and NIH/3T3 cells, using PI3 Kinase p85 (19H8) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7 and K-562 cells using PI3 Kinase p110β (C33D4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and RN33B cells using PI3 Kinase Class III (D4E2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, Jurkat and K562 cells using PI3 Kinase p110γ (D55D5) Rabbit mAb.

バックグラウンド

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).

使用例
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7074   Anti-rabbit IgG, HRP-linked Antibody
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9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

PI3 Kinase Antibody Sampler Kit

Immune Cell Signaling Pathways

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製品特集

Applications