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#9656 StemLight™ Pluripotency Antibody Kit

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希望納入価格 (円)
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2019年1月18日15時10分 現在
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Stem Cell Kit 製品一覧

9656 の推奨プロトコール i

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キット内容 容量 用途 希釈率 アイソタイプ
Oct-4A (C30A3) Rabbit mAb 100 tests IF-IC 1:200 Rabbit IgG
Sox2 (D6D9) Rabbit mAb 100 tests IF-IC 1:200 Rabbit IgG
Nanog (D73G4) XP® Rabbit mAb 100 tests IF-IC 1:200 Rabbit IgG
SSEA4 (MC813) Mouse mAb 100 tests IF-IC 1:200 Mouse IgG3
TRA-1-60(S) (TRA-1-60(S)) Mouse mAb 100 tests IF-IC 1:200 Mouse IgM
TRA-1-81 (TRA-1-81) Mouse mAb 100 tests IF-IC 1:200 Mouse IgM

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)

貯法
-20℃
社内データ

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 5 days) (right panel), using Neurofilament-L (C28E10) Rabbit mAb #2837 (green, upper), and β3-Tubulin (TU-20) Mouse mAb #4466 (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the appearance of neuronal markers and structures as cells differentiate along the neuronal lineage with retinoic acid treatment.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa cells (right) using Oct-4A (C30A3) Rabbit mAb (green, upper), Sox2 (D6D9) XP® Rabbit mAb (green, middle) and Nanog (D73G4) XP® Rabbit mAb (green, lower).

IF-IC

IF-IC

Projected confocal z-stack of human iPS cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green, upper left), TRA-1-81 (TRA-1-81) Mouse mAb (green, upper middle), SSEA4 (MC813) Mouse mAb (green, upper right), Oct-4A (C30A3) Rabbit mAb (green, lower left), Sox2 (D6D9) XP® Rabbit mAb (green, lower middle) and Nanog (D73G4) XP® Rabbit mAb (green, lower right). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 5 days) (right panel), using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green, upper), TRA-1-81 (TRA-1-81) Mouse mAb (green, middle) and SSEA4 (MC813) Mouse mAb (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the loss of pluripotency markers (green) as cells differentiate along the neuronal lineage with retinoic acid treatment.

バックグラウンド

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells.

Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated.

SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neuraminadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9).

使用文献
 
関連製品
2837   Neurofilament-L (C28E10) Rabbit mAb
2840   Oct-4A (C30A3) Rabbit mAb
3579   Sox2 (D6D9) XP® Rabbit mAb
3580   Nanog Antibody
3695   LIN28A (D84C11) XP® Rabbit mAb
3978   LIN28A (A177) Antibody
4038   KLF4 Antibody
4084   DRAQ5®
4408   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
4409   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4410   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
4412   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
4413   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4414   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
4466   β3-Tubulin (TU-20) Mouse mAb
4745   TRA-1-81 (TRA-1-81) Mouse mAb
4746   TRA-1-60(S) (TRA-1-60(S)) Mouse mAb
4755   SSEA4 (MC813) Mouse mAb
5605   c-Myc (D84C12) Rabbit mAb
5930   LIN28A (6D1F9) Mouse mAb

DRAQ5 is a registered trademark of Biostatus Limited.
StemLight™ is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

StemLight™ Pluripotency Antibody Kit

Immune Cell Signaling Pathways

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