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#97094 PhosphoPlus® ULK1 (Ser555) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
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2019年3月26日15時45分 現在
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ULK1 製品一覧

97094 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† Mol. Wt. アイソタイプ
ULK1 (D8H5) Rabbit mAb #8054 100 µl WB, IP H, M, R, Mk 150 kDa Rabbit IgG
Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb #5869 100 µl WB, IP H, M Rat 140-150 kDa Rabbit IgG

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin #9996 (0.5 μM, 30 minutes), and C2C12 cells, untreated or treated with hydrogen peroxide (10 mM, 5 minutes), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ULK1 (D8H5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin #9996 (0.5 µM, 30 minutes), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb (left). Phospho-specificity is demonstrated by pre-incubating the antibody with phosphorylated (middle) or non-phosphorylated peptides (right) against a region surrounding Ser555 of ULK1.


Western Blotting

Western Blotting

Western blot analysis of extracts from wild-type MEF and ULK1 (-/-) MEF cells using ULK1 (D8H5) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). MEF cells were kindly provided by Dr. Reuben Shaw (Salk Institute, La Jolla, CA).

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (100 μM, 2 hr; +), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower).

バックグラウンド

Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGAP and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16).

Phosphorylation of ULK1 by AMPK at Ser555 is critical for starvation-induced autophagy, cell survival under conditions of low nutrients and energy, and mitochondrial homeostasis (17).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
5869   Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
6883   SignalFire™ ECL Reagent
7727   Biotinylated Protein Ladder Detection Pack
8054   ULK1 (D8H5) Rabbit mAb

PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

PhosphoPlus® ULK1 (Ser555) Antibody Duet

Metabolic Reprogramming in Disease

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