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#97318 PhosphoPlus® TrkA (Tyr490)/TrkB (Tyr516) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
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2019年3月22日15時35分 現在
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#97318S1 Kit106,000
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TrkA 製品一覧

97318 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† Mol. Wt. アイソタイプ
Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619 100 µl WB, IP H, R Mouse 140 kDa Rabbit IgG
Trk (pan) (A7H6R) Rabbit mAb #92991 100 µl WB, IP H, M, R 120-140 kDa Rabbit IgG

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Reactivity Key: H=Human, R=Rat, M=Mouse

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells stably transfected with either TrkA or TrkB, left untreated or treated with NGF or BDNF, respectively, using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb (upper) and pooled TrkA/TrkB antibodies (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse neonatal and rat fetal brain using Trk (pan) (A7H6R) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or NGF-treated 3T3/TrkA and PC12 cells using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from M0-91, KM12 cells and mouse neonatal brain using Trk (pan) (A7H6R) Rabbit mAb.

IP

IP

Immunoprecipitation of mouse neonatal brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Trk (pan) (A7H6R) Rabbit mAb. Western blot analysis was performed using Trk (pan) (A7H6R) Rabbit mAb.

バックグラウンド

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10).

The phosphorylation sites are conserved between TrkA and TrkB: Tyr490 of TrkA corresponds to Tyr512 in TrkB, and Tyr674/675 of TrkA to Tyr706/707 in TrkB of the human sequence (14). TrkB is overexpressed in tumors, such as neuroblastoma, prostate adenocarcinoma, and pancreatic ductal adenocarcinoma (15). Research studies have shown that in neuroblastomas, overexpression of TrkB correlates with an unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF) (16-18). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms’ tumors and this isoform may act as a dominant-negative regulator of TrkB signaling (17).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
30697   TrkA (D7U3A) Rabbit mAb
3376   TrkC (C44H5) Rabbit mAb
4607   TrkB (80G2) Rabbit mAb
4619   Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb
4621   Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb
6883   SignalFire™ ECL Reagent
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
92991   Trk (pan) (A7H6R) Rabbit mAb

Tween is a registered trademark of ICI Americas, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

PhosphoPlus® TrkA (Tyr490)/TrkB (Tyr516) Antibody Duet

Metabolic Reprogramming in Disease

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