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#9769 14-3-3 Family Antibody Sampler Kit

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2019年1月17日15時10分 現在
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14-3-3 製品一覧

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
14-3-3 β/α Antibody #9636 20 µl WB H, M, R, Mk 28 Rabbit
14-3-3 γ (D15B7) Rabbit mAb #5522 20 µl WB H, M, R, Mk, Pg C, X, Z 27 Rabbit IgG
14-3-3 ζ/δ (D7H5) Rabbit mAb #7413 20 µl WB H, M, R, Mk, Pg C, B 28 Rabbit IgG
14-3-3 ε Antibody #9635 20 µl WB H, M, R, Mk 28 Rabbit
14-3-3 τ Antibody #9638 20 µl WB, IHC-P H, M, R, Mk 28 Rabbit
14-3-3 η (D23B7) Rabbit mAb #5521 20 µl WB H, M, R, Mk, B, Pg 27 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Pg=Pig, B=Bovine

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody.

Western Blotting

Western Blotting

Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.

Isoform Specificity

Isoform Specificity

Isoform specificity of 14-3-3 η (D23B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each) were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 η (D23B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).


Isoform Specificity

Isoform Specificity

Isoform specificity of 14-3-3γ (D15B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each)were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 γ (D15B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

バックグラウンド

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).

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LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

14-3-3 Family Antibody Sampler Kit

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