#9776 Myosin Light Chain 2 Antibody Sampler Kit
|Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671||20 µl||WB, IF-IC||H, M, R, Dm||C, X, Z, B, Pg||18||Rabbit|
|Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody #3674||20 µl||WB||H, M||R, C, X, Z, B, Pg||18||Rabbit|
|Myosin Light Chain 2 (D18E2) Rabbit mAb #8505||20 µl||WB||H, M, R||18||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||WB||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, R=Rat, Dm=D. melanogaster
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from HEK293 cells stimulated with ionophore A23187 for the indicated times, using Phospho-Myosin Light Chain 2 (Ser19) Antibody.
Western blot analysis of extracts from HEK293 cells stimulated with ionophore A23187 for the indicated times, using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody.
Western blot analysis of extracts from various cell lines using Myosin Light Chain 2 (D18E2) Rabbit mAb.
Confocal immunofluorescent images of HeLa cells, untreated (left) or phosphatase-treated (right), labeled with Phospho-Myosin Light Chain 2 (Ser19) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).
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- Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605.
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DRAQ5 is a registered trademark of Biostatus Limited.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
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