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#9779 Pim Kinase Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Pim-1 (C93F2) Rabbit mAb #3247 20 µl WB H, M R, Mk, B 34 Rabbit IgG
Pim-2 (D1D2) Rabbit mAb #4730 20 µl WB, IP H 40, 38, 34 Rabbit
Pim-3 (D17C9) Rabbit mAb #4165 20 µl WB H, M, R Mk 35 Rabbit IgG
Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284 20 µl WB, IHC-P, F H, M, R, Mk 23 Rabbit IgG
Bad (D24A9) Rabbit mAb #9239 20 µl WB H, M, R, Mk B 23 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb (upper) or Bad Antibody #9292 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, Raji and KARPAS-620 cell lines using Pim-2 (D1D2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pim-1 (C93F2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bad (D24A9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pim-3 (D17C9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase treated (right), using Phospho-Bad (Ser 112) (40A9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of recombinant Pim-1, Pim-2 and Pim-3 kinases using Pim-2 (D1D2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of recombinant Pim-1, -2, and -3 using Pim-1 (C93F2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of recombinant Pim-1, 2, and 3 using Pim-3 (D17C9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded COS cells untreated (left) or TPA-treated (right), showing induced cytoplasmic staining using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untransfected () or transfected with a mouse Pim-3 construct (+), using Pim-3 (D17C9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Bad (Ser 112) (40A9) Rabbit Monoclonal Antibody preincubated with control peptide (left) or Phospho-Bad (Ser 112) Blocking Peptide (IHC Specific) #1026 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma, using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells, untreated (blue) or TPA/Calyculin A treated (green), using Phospho-Bad (Ser112) (40A9) Rabbit mAb.


バックグラウンド

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).

The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability (13).

使用例
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LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Pim Kinase Antibody Sampler Kit

Immune Cell Signaling Pathways

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製品特集

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