#9780 PP2A Antibody Sampler Kit
|PP2A A Subunit (81G5) Rabbit mAb #2041||20 µl||WB, IHC-P, IF-IC||H, M, R, Mk||62||Rabbit IgG|
|PP2A B Subunit (100C1) Rabbit mAb #2290||20 µl||WB, IP, IHC-P||H, M, R, Mk, Dm||C, B, Pg||52||Rabbit IgG|
|PP2A C Subunit (52F8) Rabbit mAb #2259||20 µl||WB, IP, IHC-P, F||H, M, R, Mk, Dm||C, Pg||36, 38||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||WB||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), IP=Immunoprecipitation, F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Dm=D. melanogaster
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using PP2A C (52F8D8) Rabbit mAb.
Western blot analysis of cell lysates from HeLa, NIH/3T3, C6 and COS cells, using PP2A B Subunit (100C1) Rabbit mAb.
Western blot analysis of extracts from various cell lines using PP2A A Subunit (81G5) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PP2A C Subunit (52F8D8) Rabbit mAb in the presence of an irrelevant control peptide (left) or PP2A C Subunit Blocking Peptide #1067 (right).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PP2A B Subunit (100C1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PP2A A Subunit (81G5) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PP2A C Subunit (52F8D8) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using PP2A A Subunit (81G5) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PP2A C Subunit (52F8D8) Rabbit mAb.
Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
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Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.