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#9784 Alzheimer's Disease Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9784T1 Kit107,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
β-Amyloid (D54D2) XP® Rabbit mAb #8243 20 µl WB, IP, IF-P H 5 Rabbit IgG
Neurofilament-L (C28E10) Rabbit mAb #2837 20 µl WB, IHC-P, IF-F H, M, R 70 Rabbit IgG
Tau (Tau46) Mouse mAb #4019 20 µl WB, IHC-P, IF-P, IF-F H, M, R B 50 to 80 Mouse IgG1
BACE (D10E5) Rabbit mAb #5606 20 µl WB, IP, IF-F, IF-IC H, M, R 70 Rabbit IgG
APP/β-Amyloid (NAB228) Mouse mAb #2450 20 µl WB, IHC-P, IF-P H, Mk, B Dg, Pg 100 to 140 Mouse IgG2a
α-Synuclein (Syn204) Mouse mAb #2647 20 µl WB, IHC-P H 18 Mouse IgG2a
GSK-3α/β (D75D3) Rabbit mAb #5676 20 µl WB, IP H, M, R, Hm, Mk 51, 46 Rabbit IgG
Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 20 µl WB, IHC-P H, M, R, Mk 51 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl WB Horse

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-P=Immunofluorescence (Paraffin), IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, B=Bovine, Hm=Hamster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using Tau (Tau46) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and SK-N-MC cells, using APP/beta-Amyloid (NAB228) Mouse mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum and HeLa cells, using α-Synuclein (Syn204) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb (upper) or GSK-3α Antibody #9338 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected and BACE transfected, and rat brain extracts using BACE (D10E5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® GSK-3α/β siRNA #6301, using GSK-3α/β (D75D3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Western Blotting

Western Blotting

Western blot analysis of human Aβ-42, Aβ-40, Aβ-39, Aβ-38, and Aβ-37 peptides (5 ng) using β-Amyloid (D54D2) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using Tau (Tau46) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using APP/beta-Amyloid (NAB228) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human brain, using α-Synuclein (Syn204) Mouse mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma , untreated (left) or lambda phosphatase treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from GSK-3β (-/-) (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and wild type (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or insulin treated, using GSK-3α/β (D75D3) XP® Rabbit mAb. (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).


Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ-42 (left) and Aβ-40 (right) peptides using β-Amyloid (D54D2) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunfluorescent analysis of H4 expressing BACE (left), or H4 wild-type, (right) cells using BACE (D10E5) Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (H4 wild type and H4 cells expressing BACE were kindly provided by Dr. Giuseppina Tesco, Tufts University, Boston, MA).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse dentate gyrus stained with Tau (Tau46) Mouse mAb (red) and CREB (86B10) Mouse mAb #9104 (blue).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right).

IF-P

IF-P

Confocal immunofluorescent analysis of paraffin-embedded human Alzheimer's brain using APP/β-Amyloid (NAB228) Mouse mAb (green) and Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb #4377 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Western Blotting

Western Blotting

Western blot analysis of human Aβ-42 peptide (1 ng) and human cerebrospinal fluid (CSF) of an AD patient using β-Amyloid (D54D2) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of mouse hippocampus using BACE (D10E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-P

IF-P

Confocal immunofluorescent analysis of human Alzheimer brain (A) labeled with Tau (Tau46) Mouse mAb (green) and APP Antibody #2452 (red) compared to normal human brain (B).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb in the presence of control peptide (left) or Phospho-GSK-3α (Ser21) (36E9) Blocking Peptide #1027 (right).

IF-F

IF-F

Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-P

IF-P

Confocal immunofluorescent analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (D54D2) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

バックグラウンド

Alzheimer's Disease (AD) is one of the most common neurodegenerative diseases worldwide. Clinically, it is characterized by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles, which results in neuronal dysfunction and cell death. Central to this disease is the differential processing of the integral transmembrane glycoprotein Amyloid β (A4) precursor protein (APP) that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of AD patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (2). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (3). The extracellular deposition and accumulation of the released Aβ fragments and an α-synuclein fragment known as the non- Aβ fragment, form the main components of amyloid plaques in AD. GSK-3α regulates the production of Aβ peptides. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates Aβ production by specifically inhibiting the cleavage of APP by γ-secretase, thereby blocking accumulation of Aβ peptides in the brains of mice that overproduce APP (4). AD is also characterized by the presence of neurofibrillary tangles. These tangles are the result of hyperphosphorylation and oligomerization of the microtubule associated protein Tau and lead to apoptosis of the neuron. In particular, phosphorylation of Tau Ser396 by GSK-3 or CDK5 destabilizes microtubules in AD (5,6). Additionally, neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM) and heavy (NFH) subunits (7). Accumulation of neurofilaments are found in many human neurological disorders including AD (7).

使用例
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2642   α-Synuclein Antibody
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2835   Neurofilament-L (DA2) Mouse mAb
2836   Neurofilament-H (RMdO 20) Mouse mAb
2838   Neurofilament-M (RMO 14.9) Mouse mAb
3622   Presenilin 1 Antibody
3823   Phospho-APP (Thr668) Antibody
3976   TACE Antibody
4147   Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb
4542   MAP2 Antibody
4818   GSK-3α (D80D1) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7722   Blue Loading Buffer Pack
8112   SignalStain® Antibody Diluent
9315   GSK-3β (27C10) Rabbit mAb
9322   Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb
9337   Phospho-GSK-3α (Ser21) (46H12) Mouse mAb
9338   GSK-3α Antibody
9632   Phospho-Tau (Ser396) (PHF13) Mouse mAb
9910   Phospho-MAPK Family Antibody Sampler Kit
9999   Nonfat Dry Milk

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Alzheimer's Disease Antibody Sampler Kit

Metabolic Reprogramming in Disease

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