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#9787 Sirtuin Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月25日15時35分 現在
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#9787T1 Kit99,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-SirT1 (Ser47) Antibody #2314 20 µl WB, IP, IF-IC, F H 120 Rabbit
SirT1 (D1D7) Rabbit mAb #9475 20 µl WB, IF-IC H, M, R, Mk C, B, Pg, Hr 120 Rabbit IgG
SirT2 (D4O5O) Rabbit mAb #12650 20 µl WB H, M, R, Mk 39, 43 Rabbit IgG
SirT3 (D22A3) Rabbit mAb #5490 20 µl WB, IP H, M, R 28 Rabbit IgG
SirT5 (D8C3) Rabbit mAb #8782 20 µl WB H, M, R 30 Rabbit IgG
SirT6 (D8D12) Rabbit mAb #12486 20 µl WB, IP, IF-IC H, M, R, Mk 42, 36 Rabbit IgG
SirT7 (D3K5A) Rabbit mAb #5360 20 µl WB, IP H, M, R, Mk 45 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of 293 cell lysates treated or untreated with λ phosphatase, using Phospho-SirT1 (Ser47) Antibody #2314 (upper) or SirT1 Antibody #2310 (lower).

Western Blotting

Western Blotting

Western blot analysis of mitochondrial extracts from SirT3 wild-type (WT) and knockout (KO) mouse liver, and of whole cell extracts from rat liver and human kidney, using SirT3 (D22A3) Rabbit mAb (upper) and AIF (D39D2) XP® Rabbit mAb #5318 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT1 (D1D7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, expressing either nontargeting shRNA (293 shNT) or shRNA targeting SirT5 (293 shSirT5), and HCT 116, Raw 264.7, and H-4-II-E cells using SirT5 (D8C3) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 293 shNT and 293 shSirT5 cells were kindly provided by David Lombard, University of Michigan.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT7 (D3K5A) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT6 (D8D12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT2 (D4O5O) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells, λ phosphatase treated (blue) or untreated (green), using Phospho-SirT1 (Ser47) Antibody compared to a nonspecific negative control antibody (red).


Western Blotting

Western Blotting

Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT1 (D1D7) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, expressing either non-targeting shRNA (shNT) or shSirT6, using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from SirT2 wild-type (WT) and knockout (KO) mouse brain using SirT2 (D4O5O) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). SirT2 WT and KO mouse brain extracts were kindly provided by Dr. Gizem Donmez, Tufts University School of Medicine.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-1080 cells, untreated (left) and phosphatase-treated (right), using Phospho-SirT1 (Ser47) Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa (positive; left), WT MEF (positive; middle), and SirT1 KO MEF (right) cells using SirT1 (D1D7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.

Western Blotting

Western Blotting

Western blot analysis of extracts from SirT6 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). SirT6 WT and KO MEF were kindly provided by Dr. David Lombard, University of Michigan.


IP

IP

Immunoprecipitation of SirT6 from HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3) or SirT6 (D8D12) Rabbit mAb (lane 2). Lane 1 is 10% input. Western blot analysis was performed using SirT6 (D8D12) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT 116 cells, expressing either non-targeting shRNA (shNT) (left) or shSirT6 (right), using SirT6 (D8D12) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

バックグラウンド

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. SirT2, one of several mammalian Sir2 homologs, deacetylates α-tubulin on Lys40 and histone H4 on Lys16, and is implicated in cytoskeletal regulation and progression through mitosis (2,3). SirT2 protein is mainly cytoplasmic and is associated with microtubules and the HDAC6 tubulin deacetylase (2).

SirT3 exists in human cells in two forms, including a full-length, nuclear (44 kDa) protein and a processed (28 kDa) protein found exclusively in the mitochondria (4-6). Full-length SirT3 protein is processed in the mitochondrial matrix by mitochondrial matrix processing peptidase (MMP) (5). Both full-length and processed SirT3 are active enzymes that deacetylate histone H3 at Lys9 and histone H4 at Lys16 in vitro (4). SirT3 also deacetylates Lys642 of acetyl-CoA synthetase 2 (AceCS2) and activates AceCS2 activity in the mitochondria (7).

SirT5 is localized to the mitochondria and has been implicated in the regulation of cell metabolism (8,9). Nuclear SirT6 is a chromatin-associated protein that promotes normal maintenance of genome integrity as mediated by the base excision repair (BER) pathway (10-12). Mammalian SirT7 is localized to the nucleolus and is prominently expressed in hematopoietic cells, especially myeloid progenitor cells (13). SirT7 is recruited to chromatin by sequence-specific DNA binding transcription factors such as Elk-4, where it facilitates transcriptional repression through deacetylation of histone H3 at Lys18 (14).

使用例
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XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Sirtuin Antibody Sampler Kit

Immune Cell Signaling Pathways

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製品特集

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