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#97999 Phospho-Jak Family Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年3月20日11時40分 現在
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Jak 製品一覧

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Jak1 (6G4) Rabbit mAb #3344 20 µl WB, IHC-P H, M, R Mk, Dg, Pg 130 Rabbit IgG
Phospho-Jak1(Tyr1034/1035) (D7N4Z) Rabbit mAb #74129 20 µl WB H, M 130 Rabbit IgG
Jak2 (D2E12) XP® Rabbit mAb #3230 20 µl WB, IP, IHC-P, IF-IC, F H, M, R Hm, Mk, C, X, B, Dg, Pg, Hr 125 Rabbit IgG
Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb #8082 20 µl WB H, M R, Mk, X, B, Pg 125 Rabbit IgG
Jak3 (D1H3) Rabbit mAb #8827 20 µl WB, IP H Mk 115 Rabbit IgG
Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb #5031 20 µl WB H, M Mk 115 Rabbit IgG
Tyk2 (D4I5T) Rabbit mAb #14193 20 µl WB, IP H, Mk 134 Rabbit IgG
Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb #68790 20 µl WB H M, R 134 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from ACHN, SR, CTLL-2 and PC12 cell lines using Jak1 (6G4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.


Western Blotting

Western Blotting

Western blot analysis of extracts from NK-92 cells, untreated or treated with Human Interleukin-2 (hIL-2) #8907 (10 ng/ml, 15 minutes), using Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb (upper) or Jak3 Antibody #3775 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from BaF3 cells, untreated or treated with Mouse Interleukin-3 (mIL-3) #8923 (10 ng/ml, 5 min), using Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb (upper) or total Jak2 (D2E12) XP® Rabbit mAb #3230 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Jak3 (D1H3) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Tyk2 (D4I5T) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved U266 and Jurkat cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (50 ng/ml, 15 min; +) using Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb (upper) or total Tyk2 (D4I5T) Rabbit mAb #14193 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, serum-starved overnight (-) followed by treatment with Human Interferon-α1 #8927 (hIFN-α1, 10 ng/ml, 15 min; +) or Human Interleukin-4 #8919 (hIL-4, 10 ng/ml, 10 min; +) using Phospho-Jak1 (Tyr1034/1035) (D7N4Z) Rabbit mAb (upper) or Jak1 (6G4) Rabbit mAb (lower).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb #3344, in the presence of control peptide (left) or Jak1 Blocking Peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from SET-2 cells, untreated or treated with the Jak2 inhibitor FLLL31 (10 μM, 4 hr), using Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb (upper) or total Jak2 (D2E12) XP® Rabbit mAb #3230 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Tyk2 (hTyk2; +), using Tyk2 (D4I5T) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP cells, untreated or treated with growth hormone (GH) (100 ng/ml, 5 min), using Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb (upper) or total Jak2 (D2E12) XP® Rabbit mAb #3230 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Tyk2 siRNA #14254 (+), using Tyk2 (D4I5T) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Tyk2 (D4I5T) Rabbit mAb confirms silencing of Tyk2 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak1 (6G4) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).


バックグラウンド

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phosphotyrosine-binding domains.

Activation of Jak kinases upon cytokine receptor binding is associated with tyrosine phosphorylation within their activation loops, including Tyr1034/1035 of Jak1, Tyr1007/1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2. Many studies have indicated that various cytokine receptors have clear preferences that utilize distinct Jak family members. Aberrant regulation of Jak signaling is associated with a number of diseases, including myeloproliferative neoplasms, leukemia, and inflammatory disease (6).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
14193   Tyk2 (D4I5T) Rabbit mAb
3230   Jak2 (D2E12) XP® Rabbit mAb
3344   Jak1 (6G4) Rabbit mAb
5031   Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb
68790   Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
74129   Phospho-Jak1(Tyr1034/1035) (D7N4Z) Rabbit mAb
7727   Biotinylated Protein Ladder Detection Pack
8082   Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb
8827   Jak3 (D1H3) Rabbit mAb

Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.
Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Phospho-Jak Family Antibody Sampler Kit

Immune Cell Signaling Pathways

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