CST logo

#98322 Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit II

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年3月20日11時40分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#98322T1 Kit115,000
ログインすると国内在庫状況がご確認いただけます。

Bcl-2 製品一覧

98322 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Bax (D2E11) Rabbit mAb #5023 20 µl WB, IP, IHC-P H 20 Rabbit IgG
Bak (D4E4) Rabbit mAb #12105 20 µl WB, IP, IHC-P, IF-IC, F H, M, R, Mk 25 Rabbit IgG
Bok (D7V2N) Rabbit mAb #86875 20 µl WB H 22 Rabbit IgG
Bim (C34C5) Rabbit mAb #2933 20 µl WB, IP, IHC-Bond, IHC-P, IF-IC, F H, M, R Mk, B, Dg, Pg 12, 15, 23 Rabbit IgG
Bad (D24A9) Rabbit mAb #9239 20 µl WB H, M, R, Mk B 23 Rabbit IgG
BID Antibody (Human Specific) #2002 20 µl WB, IP H 15, 22 Rabbit
Puma (D30C10) Rabbit mAb #12450 20 µl WB, IP H 23 Rabbit IgG
Noxa (D8L7U) Rabbit mAb #14766 20 µl WB, IP H 10 Rabbit IgG
Bik Antibody #4592 20 µl WB H 20 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IHC-Bond=IHC-Leica® Bond™
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM), and RS4;11 cells, untreated or okadaic acid-treated (1 µM), using BID Antibody (Human Specific).

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji, A20 and RL-7 cells using Bim (C34C5) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from Raji and Ramos cell lines using Bik Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bad (D24A9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bax (D2E11) Rabbit mAb. Brimmel et al. demonstated that Jurkat cells lack Bax protein expression (10).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bak (D4E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, untreated (-) or treated with Doxorubicin #5927 (500 nM, overnight; +), using Puma (D30C10) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from KARPAS-299, RL-7, and L-540 cells lines using Noxa (D8L7U) Rabbit mAb. Cell Line Source: Dr. Abraham Karpas at the University of Cambridge.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bok (D7V2N) Rabbit mAb.

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Bim (C34C5) Rabbit mAb performed on the Leica® BOND™ Rx.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Bim siRNA I #6461 or SignalSilence® Bim siRNA II (+), using Bim (C34C5) Rabbit mAb #2933 and α-Tubulin (11H10) Rabbit mAb #2125. Bim (C34C5) Rabbit mAb confirms silencing of Bim expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bim siRNA.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Bax (D2E11) Rabbit mAb in the presence of control peptide (upper) or antigen-specific peptide (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Bak (D4E4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Puma (D30C10) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved Jurkat cells, untreated (-) or treated with TPA #4174 (10 ng/ml, 3 hr; +), using Noxa (D8L7U) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Bok (hBok-Myc/DDK; +) using Bok (D7V2N) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bim (C34C5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Bak (D4E4) Rabbit mAb.

IP

IP

Immunoprecipitation of Puma from RL-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Puma (D30C10) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Puma (D30C10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Noxa protein (hNoxa; +), using Noxa (D8L7U) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using Bim (C34C5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse prostate using Bak (D4E4) Rabbit mAb.

IP

IP

Immunoprecipitation of Noxa protein from KARPAS-299 cells using Rabbit (DA1E) mAb XP® Isotype Control #3900 (lane 2) or Noxa (D8L7U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Noxa (D8L7U) Rabbit mAb. Cell Line Source: Dr. Abraham Karpas at the University of Cambridge.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Bim (C34C5) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SKBR3 cells using Bak (D4E4) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raji cells using Bim (C34C5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of OVCAR8 cells using Bak (D4E4) Rabbit mAb (green; left) showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos (red; center). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using Bim Antibody (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok; and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12105   Bak (D4E4) Rabbit mAb
12450   Puma (D30C10) Rabbit mAb
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
14766   Noxa (D8L7U) Rabbit mAb
2002   BID Antibody (Human Specific)
2933   Bim (C34C5) Rabbit mAb
4592   Bik Antibody
5023   Bax (D2E11) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
86875   Bok (D7V2N) Rabbit mAb
9239   Bad (D24A9) Rabbit mAb

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
MitoTracker is a registered trademark of Molecular Probes, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit II

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications