CST logo

#9839 AMPK Subunit Antibody Sampler Kit

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年1月18日15時10分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#9839T1 Kit99,000
ログインすると国内在庫状況がご確認いただけます。

AMPK 製品一覧

9839 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
AMPKα1 Antibody #2795 20 µl WB H, Mk Pg 62 Rabbit
AMPKα2 Antibody #2757 20 µl WB, IP H, Mk 62 Rabbit
AMPKβ1 (71C10) Rabbit mAb #4178 20 µl WB H, M, R, Hm, Mk, Pg 38 Rabbit IgG
AMPKβ2 Antibody #4148 20 µl WB, IP H, M, R, Mk 30 Rabbit
AMPKγ1 Antibody #4187 20 µl WB H, Mk 37 Rabbit
AMPKγ2 Antibody #2536 20 µl WB H, M, R, Mk, B 75 Rabbit
AMPKγ3 Antibody #2550 20 µl WB H 54 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, Mk=Monkey, M=Mouse, R=Rat, Hm=Hamster, Pg=Pig, B=Bovine

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 (human), NBT-II (rat), and Neuro-2A (mouse) cells, using AMPKγ2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and K562 cells, using AMPKgamma1 Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293, C6, L929 and COS cells, using AMPKβ2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from RD cells, using AMPKγ3 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, HT29 and COS cells, using AMPKα1 Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 and COS cells, using AMPKα2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using AMPKβ1 (71C10) Rabbit mAb.

IP

IP

Immunprecipitation of AMPK alpha 2 from 293 cell extracts using AMPK alpha 2 antibody (Lane 1). Lane 2: No antibody control.


バックグラウンド

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).

使用文献
 
関連製品
2531   Phospho-AMPKα (Thr172) Antibody
2532   AMPKα Antibody
2537   Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb
2793   AMPKα (F6) Mouse mAb
2795   AMPKα1 Antibody
4184   Phospho-AMPKα1 (Ser485) Antibody
4185   Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody
4186   Phospho-AMPKβ1 (Ser182) Antibody
4188   Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb
7075   Anti-biotin, HRP-linked Antibody
9944   AICAR
9996   Oligomycin
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

AMPK Subunit Antibody Sampler Kit

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications