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#9868 CDK Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月18日15時10分 現在
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#9868T1 Kit92,000
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CDK 製品一覧

9868 の推奨プロトコール i

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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
cdc2 (POH1) Mouse mAb #9116 20 µl WB, IP, IF-IC H, Mk 34 Mouse IgG2a
CDK2 (78B2) Rabbit mAb #2546 20 µl WB, IP, F H, M, R, Mk 33 Rabbit
CDK4 (D9G3E) Rabbit mAb #12790 20 µl WB, IHC-P, IF-IC, F H, Mk 30 Rabbit IgG
CDK6 (D4S8S) Rabbit mAb #13331 20 µl WB, IP H, Mk 36 Rabbit IgG
CDK7 (MO1) Mouse mAb #2916 20 µl WB, IP, IHC-P H, R 40 Mouse IgG2b
CDK9 (C12F7) Rabbit mAb #2316 20 µl WB, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Hm, Mk, B, Dg 42, 55 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl WB Horse

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human, Mk=Monkey, M=Mouse, R=Rat, Hm=Hamster, B=Bovine, Dg=Dog

貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using cdc2 (POH1) Mouse mAb.

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC and C6 cells, using CDK7 (MO1) Mouse mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using CDK2 (78B2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using CDK9 (C12F7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using CDK7 (MO1) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using CDK2 (78B2) Rabbit mAb versus propidium iodide (DNA content).


IP

IP

Immunoprecipitation of CDK9 from HeLa cells using CDK9 (C12F7) Rabbit mAb. Western blot detection was performed using the same antibody. Lane 1 is 5% input.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK4 (D9G3E) Rabbit mAb.

IP

IP

Immunoprecipitation of CDK6 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CDK6 (D4S8S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CDK6 (DCS83) Mouse mAb #3136.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left), SignalSilence® cdc2 siRNA I #3500 (center) or SignalSilence® cdc2 siRNA II #3600 (right), using cdc2 (POH1) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK9 (C12F7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CDK4 (D9G3E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded K7M2 mouse syngeneic tumor using CDK9 (C12F7) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using CDK4 (D9G3E) Rabbit mAb and Propidium Iodid (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen SKOV-3 xenograft using CDK9 (C12F7) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using CDK4 (D9G3E) Rabbit mAb (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8493 (red), and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #3458 (blue pseudocolor).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using CDK9 (C12F7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using CDK9 (C12F7) Rabbit mAb (green). Actin filaments have been labeled with DY-555 phalloidin (red).


バックグラウンド

Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7).

使用例
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6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9869   Cyclin Antibody Sampler Kit

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

CDK Antibody Sampler Kit

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製品特集

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